Evidence map›Paper›PMID 36273137›Full record

ArticleBMC genomics2022

Transcriptome and population structure of glassy-winged sharpshooters (Homalodisca vitripennis) with varying insecticide resistance in southern California.

Cassandra L Ettinger, Frank J Byrne, Inaiara de Souza Pacheco, Dylan J Brown, Linda L Walling, Peter W Atkinson, Richard A Redak, Jason E Stajich

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.3field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Cassandra L EttingerDepartment of Microbiology and Plant Pathology, University of California, Riverside, Riverside, CA, USA. cassande@ucr.edu.
Frank J ByrneDepartment of Entomology, University of California, Riverside, Riverside, CA, USA.
Inaiara de Souza PachecoDepartment of Entomology, University of California, Riverside, Riverside, CA, USA.
Dylan J BrownDepartment of Entomology, University of California, Riverside, Riverside, CA, USA.
Linda L WallingDepartment of Botany and Plant Sciences, University of California, Riverside, Riverside, CA, USA.
Peter W AtkinsonDepartment of Entomology, University of California, Riverside, Riverside, CA, USA.
Richard A RedakDepartment of Entomology, University of California, Riverside, Riverside, CA, USA.
Jason E StajichDepartment of Microbiology and Plant Pathology, University of California, Riverside, Riverside, CA, USA. jason.stajich@ucr.edu.
University of California, Riverside · US

Funding

California Department of Food and Agriculture 007011-003California Department of Food and Agriculture 20-0267National Institute of Food and Agriculture CA-R-PPA-5062-HPierce's Disease Control program 14-0379-000-SA-2
6 · The paper itself

Abstract

backgroundHomalodisca vitripennis Germar, the glassy-winged sharpshooter, is an invasive insect in California and a critical threat to agriculture through its transmission of the plant pathogen, Xylella fastidiosa. Quarantine, broad-spectrum insecticides, and biological control have been used for population management of H. vitripennis since its invasion and subsequent proliferation throughout California. Recently wide-spread neonicotinoid resistance has been detected in populations of H. vitripennis in the southern portions of California's Central Valley. In order to better understand potential mechanisms of H. vitripennis neonicotinoid resistance, we performed RNA sequencing on wild-caught insecticide-resistant and relatively susceptible sharpshooters to profile their transcriptome and population structure.

resultsWe identified 81 differentially expressed genes with higher expression in resistant individuals. The significant largest differentially expressed candidate gene linked to resistance status was a cytochrome P450 gene with similarity to CYP6A9. Furthermore, we observed an over-enrichment of GO terms representing functions supportive of roles in resistance mechanisms (cytochrome P450s, M13 peptidases, and cuticle structural proteins). Finally, we saw no evidence of broad-scale population structure, perhaps due to H. vitripennis' relatively recent introduction to California or due to the relatively small geographic scale investigated here.

conclusionsIn this work, we characterized the transcriptome of insecticide-resistant and susceptible H. vitripennis and identified candidate genes that may be involved in resistance mechanisms for this species. Future work should seek to build on the transcriptome profiling performed here to confirm the role of the identified genes, particularly the cytochrome P450, in resistance in H. vitripennis. We hope this work helps aid future population management strategies for this and other species with growing insecticide resistance.

Indexed as

HemipteraInsecticidesAnimalsCytochromesInsecticide ResistanceNeonicotinoidsPeptide HydrolasesTranscriptomeCytochromesInsecticidesNeonicotinoidsPeptide HydrolasesCytochrome P450sDifferentially expressed genesGlassy-winged sharpshooterHomalodisca vitripennisInsecticide resistanceNeonicotinoidsRNA-seqTranscriptome

Identifiers

PMID36273137
PMCPMC9587601
OpenAlexW4307066818

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.