ArticleScientific reports2022
Environmental RNA as a Tool for Marine Community Biodiversity Assessments.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed.
- Biomonitoring 3.0: Beyond Taxa Lists to Evidence-Tiered Monitoring of Ecosystem Dynamics and Interactions.Ecology and evolution · 2026Review
- Prospects of multipurpose biomonitoring for fisheries assessment based on environmental nucleic acids.Journal of fish biology · 2026Review
- A field-deployable CRISPR-based biosensing platform for monitoring marine ecosystems.Nature sustainability · 2026Article
- Pattern of Detections Across Multiple Environmental Messenger RNAs (e-mRNAs) in Stressor-Exposed Zebrafish (Ecology and evolution · 2026Article
- DeepCOI: a large language model-driven framework for fast and accurate taxonomic assignment in animal metabarcoding.Genome biology · 2025Article
- The use of environmental RNA for inferring fish spawning behavior.Scientific reports · 2025Article
- Contrasting diversity patterns between microeukaryotic and prokaryotic communities in cold-seep sediments.ISME communications · 2025Article
- Nucleic Acid Based Testing (NABing): A Game Changer Technology for Public Health.Molecular biotechnology · 2024Review
- Evaluating eDNA and eRNA metabarcoding for aquatic biodiversity assessment: From bacteria to vertebrates.Environmental science and ecotechnology · 2024Article
- Sediment Toxicity Tests: A Critical Review of Their use in Environmental Regulations.Environmental toxicology and chemistry · 2024Review
- Measuring the state of aquatic environments using eDNA-upscaling spatial resolution of biotic indices.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Article
- New prospects of environmental RNA metabarcoding research in biological diversity, ecotoxicological monitoring, and detection of COVID-19: a critical review.Environmental science and pollution research international · 2024Review
- Article
- Using eRNA/eDNA metabarcoding to detect community-level impacts of nanoplastic exposure to benthic estuarine ecosystems.Environmental pollution (Barking, Essex : 1987) · 2023Article
- Freshwater Haplosporidian Diversity Associated With Invasive Non-Native Bivalves in the River Thames, Including Haplosporidium raabei Infecting Dreissena polymorpha.The Journal of eukaryotic microbiologyArticle
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microscopic organisms are often overlooked in traditional diversity assessments due to the difficulty of identifying them based on morphology. Metabarcoding is a method for rapidly identifying organisms where Environmental DNA (eDNA) is used as a template. However, legacy DNA is problematically detected from organisms no longer in the environment during sampling. Environmental RNA (eRNA), which is only produced by living organisms, can also be collected from environmental samples and used for metabarcoding. The aim of this study was to determine differences in community composition and diversity between eRNA and eDNA templates for metabarcoding. Using mesocosms containing field-collected communities from an estuary, RNA and DNA were co-extracted from sediment, libraries were prepared for two loci (18S and COI), and sequenced using an Illumina MiSeq. Results show a higher number of unique sequences detected from eRNA in both markers and higher α-diversity compared to eDNA. Significant differences between eRNA and eDNA for all β-diversity metrics were also detected. This study is the first to demonstrate community differences detected with eRNA compared to eDNA from an estuarine system and illustrates the broad applications of eRNA as a tool for assessing benthic community diversity, particularly for environmental conservation and management applications.
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Registered trials
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