ArticleScientific reports2022
Hydrogel-based microfluidic device with multiplexed 3D in vitro cell culture.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Microfluidic diffusional sizing in bioanalysis and biosensing.Lab on a chip · 2026Review
- A pump-free microfluidic device for integrated multi-functional testing of tumor spheroids.APL bioengineering · 2026Article
- Rapid hydrogel micropatterning utilizing CNT-induced thermoresponsive effect.Scientific reports · 2026Article
- Improving Diffusion in Collagen Hydrogels for 3D Culture of Rat Cardiac or Dermal Fibroblasts via Magnetically Actuated Vibrating Microparts.Gels (Basel, Switzerland) · 2026Article
- Harnessing Next-Generation 3D Cancer Models to Elucidate Tumor-Microbiome Crosstalk.Advanced healthcare materials · 2026Review
- Artificial Intelligence-Aided Microfluidic Cell Culture Systems.Biosensors · 2025Review
- Review
- From Basic to Breakthroughs: The Journey of Microfluidic Devices in Hydrogel Droplet Generation.Gels (Basel, Switzerland) · 2025Review
- Mechanical interaction between a hydrogel and an embedded cell in biomicrofluidic applications.Biomicrofluidics · 2025Article
- Overview of research on additive manufacturing of hydrogel-assisted lab-on-chip platforms for cell engineering applications in photodynamic therapy research.Mikrochimica acta · 2024Review
- Review
- A Versatile Photocrosslinkable Silicone Composite for 3D Printing Applications.Advanced materials technologies · 2024Article
- Microfluidics in High-Throughput Drug Screening: Organ-on-a-Chip andBiosensors · 2024Review
- Review
- 3D bio-printed hydrogel inks promoting lung cancer cell growth in a lab-on-chip culturing platform.Mikrochimica acta · 2023Article
- Fabrication of a Low-Cost Microfluidic Device for High-Throughput Drug Testing on Static and Dynamic Cancer Spheroid Culture Models.Diagnostics (Basel, Switzerland) · 2023Article
- A Silicon-Based PDMS-PEG Copolymer Microfluidic Chip for Real-Time Polymerase Chain Reaction Diagnosis.Journal of functional biomaterials · 2023Article
- Recent Progress and Perspectives on Neural Chip Platforms Integrating PDMS-Based Microfluidic Devices and Microelectrode Arrays.Micromachines · 2023Review
- Breaking the clean room barrier: exploring low-cost alternatives for microfluidic devices.Frontiers in bioengineering and biotechnology · 2023Review
- Optimization of 3D-aggregated spheroid model (3D-ASM) for selecting high efficacy drugs.Scientific reports · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microfluidic devices that combine an extracellular matrix environment, cells, and physiologically relevant perfusion, are advantageous as cell culture platforms. We developed a hydrogel-based, microfluidic cell culture platform by loading polyethylene glycol (PEG) hydrogel-encapsulated U87 glioblastoma cells into membrane-capped wells in polydimethyl siloxane (PDMS). The multilayer microfluidic cell culture system combines previously reported design features in a configuration that loads and biomimetically perfuses a 2D array of cell culture chambers. One dimension of the array is fed by a microfluidic concentration gradient generator (MCGG) while the orthogonal dimension provides loading channels that fill rows of cell culture chambers in a separate layer. In contrast to typical tree-like MCGG mixers, a fractional serial dilution of 1, ½, ¼, and 0 of the initial solute concentration is achieved by tailoring the input microchannel widths. Hydrogels are efficiently and reproducibly loaded in all wells and cells are evenly distributed throughout the hydrogel, maintaining > 90% viability for up to 4 days. In a drug screening assay, diffusion of temozolomide and carmustine to hydrogel-encapsulated U87 cells from the perfusion solution is measured, and dose-response curves are generated, demonstrating utility as an in vitro mimic of the glioblastoma microenvironment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.