ArticleScientific reports2022
Optimization, purification, and characterization of xylanase production by a newly isolated Trichoderma harzianum strain by a two-step statistical experimental design strategy.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 62 citations in OpenAlex.
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- Cell-Surface and Periplasmic Deconstruction of Xylan by Oligotrophic Bacterium Caulobacter vibrioides.Environmental microbiology · 2026Article
- Whole-genome analysis and enzymatic profiling of Bacillus spizizenii as a xylanase-producing source from Iran.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Integrated bioprocess optimization of cellulase and xylanase production by Penicillium argillaceum using lignocellulosic agro-industrial residues.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Multi-enzyme producing lignocellulolytic bacteria from biogas slurry: molecular identification and optimization of enzyme production by Bacillus subtilis BCA-1.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Solid-State Fermentation of Green Coconut Husk byACS omega · 2026Article
- Utilization of sugarcane bagasse waste by Aspergillus welwitchiae SVUAw9 for production, purification, and characterization of cold-active xylanase under solid-state fermentation conditions.Microbial cell factories · 2026Article
- Screening of a xylanase-producing Trichoderma strain and optimization of its enzyme production conditions.Biotechnology letters · 2026Article
- EMS-induced mutagenesis of Aspergillus terreus for enhanced xylanase production and subsequent biotransformation of sugarcane bagasse-derived xylose towards xylitol.Archives of microbiology · 2026Article
- Study of Fermentation Conditions Optimization for Xylanase Production byFoods (Basel, Switzerland) · 2026Article
- Enhanced Mesophilic Degradation of Rice Straw by Microbial Consortium SXJG15 Through Coordinated Enzymatic Activity and Community Reshaping.Microorganisms · 2025Article
- Enzyme Technology in the Food Industry: Molecular Mechanisms, Applications, and Sustainable Innovations.Food science & nutrition · 2025Review
- Insight into the eminent biotechnological applications of xylanolytic enzymes for sustainable bioprocessing.3 Biotech · 2025Review
- Efficient Assessment and Optimisation of Medium Components Influencing Extracellular Xylanase Production byInternational journal of molecular sciences · 2025Article
- Biochemical Characterization of a GH11 Xylanase from Xylanase-Producing Trichoderma citrinoviride.Applied biochemistry and biotechnology · 2025Article
- Isolation, expression, and in silico profiling of a thermostable xylanase from Geobacillus stearothermophilus strain NASA267: insights into structural features and agro-waste valorization.Microbial cell factories · 2025Article
- Engineering the green algae Chlamydomonas incerta for recombinant protein production.PloS one · 2025Article
- Establishing the green algaebioRxiv : the preprint server for biology · 2024Article
- Genome Characteristics of the Endophytic FungusJournal of fungi (Basel, Switzerland) · 2024Article
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4 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Xylanases are hydrolytic enzymes with a wide range of applications in several industries such as biofuels, paper and pulp, food, and feed. The objective of this study was to optimize the culture conditions and medium components for maximal xylanase production from a newly isolated Trichoderma harzianum strain using the Plackett-Burman Design (PBD) and Box Behnken Design (BBD) experimental strategies. Xylanase production was enhanced 4.16-fold to 153.80 U/ml by BBD compared to a preliminary one-factor-at-a-time (OFAT) activity of 37.01 U/ml and 2.24-fold compared to the PBD (68.70 U/ml). The optimal conditions for xylanase production were: 6 days of fermentation, incubation temperature of 70 °C, pH 5.0, agitation of 160 rpm, and 1.2% wheat bran and ammonium sulphate. The experimental design effectively provided conditions for the production of an acidic-thermostable enzyme with exciting potential for application in animal feed improvement. The acidic-thermostable xylanase was purified from the submerged culture and SDS-PAGE analysis revealed a molecular weight of 72 kDa. This protein had maximum xylanolytic activity at pH 6.0 and 65 °C and was stable for 4 h retaining > 70% activity and exhibited substrate specificity for beechwood xylan with a K
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