Evidence map›Paper›PMID 36272086›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

A Practical Guide for CRISPR-Cas9-Induced Mutations in Axolotls.

Konstantinos Sousounis, Katharine Courtemanche, Jessica L Whited

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.0field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Konstantinos SousounisDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
Katharine CourtemancheDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.
Jessica L WhitedDepartment of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA. jessica_whited@harvard.edu.
Harvard University · USHarvard Stem Cell Institute · US

Funding

Leveraging Single-Cell Analysis to Elucidate Mechanisms of Vertebrate LimbRegenerationDP2HD087953 · NICHD · HARVARD UNIVERSITY · PI WHITED, JESSICA L. · 2015 to 2020
$3.2M
IDENTIFYING ROADBLOCKS TO LIMB REGENERATIONR01HD095494 · NICHD · HARVARD UNIVERSITY · PI WHITED, JESSICA L. · 2019 to 2023
$1.9M
Elucidation of cellular reprogramming processes that drive lens regeneration in axolotl as a basis for future therapeutic approachesK99EY029361 · NEI · HARVARD UNIVERSITY · PI SOUSOUNIS, KONSTANTINOS · 2019 to 2020
$257k
NEI NIH HHS K99 EY029361NICHD NIH HHS DP2 HD087953NICHD NIH HHS R01 HD095494
6 · The paper itself

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR) is a powerful tool that enables editing of the axolotl genome. In this chapter, we will cover how to retrieve gene sequences, confirm annotation, design CRISPR targets, analyze indels, and screen for Crispant axolotls. This is a comprehensive guide on how to use CRISPR on your favorite gene and gain insights into its function.

Indexed as

CRISPR-Cas SystemsGene EditingAmbystoma mexicanumAnimalsGenomeMutationRNA, Guide, CRISPR-Cas SystemsRNA, Guide, CRISPR-Cas SystemsAxolotlsAxolotl screeningCRISPRCRISPR-Cas9CRISPR designGenome targeting

Identifiers

PMID36272086
PMCPMC12338337
OpenAlexW4307097250

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.