ArticleBMC microbiology2022
Visual LAMP method for the detection of Vibrio vulnificus in aquatic products and environmental water.
Article in BMC microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- PfAgo-Enhanced LAMP Assay for Rapid and Specific Detection ofFoods (Basel, Switzerland) · 2026Article
- Environmental Application of Newly Designed Loop-Mediated Isothermal Amplification (LAMP) Kits for Nosocomial Pathogen Detection in Hospital Settings.Life (Basel, Switzerland) · 2026Article
- Improving Microbiological Monitoring of Hospital Surfaces: Loop-Mediated Isothermal Amplification (LAMP) as a New Approach for Rapid Nosocomial Pathogens Detection.International journal of environmental research and public health · 2026Article
- Comparative evaluation of 14Frontiers in microbiology · 2026Article
- Public health aspects ofEFSA journal. European Food Safety Authority · 2024Article
- Isolation ofMicroorganisms · 2024Article
- Evaluation of a novel lysis-based sample processing method to optimize Vibrio vulnificus detecting by loop-mediated isothermal amplification assay.BMC microbiology · 2024Article
- Rapid visual nucleic acid detection of Vibrio alginolyticus by recombinase polymerase amplification combined with CRISPR/Cas13a.World journal of microbiology & biotechnology · 2023Article
- A Novel RAA Combined Test Strip Method Based on Dual Gene Targets for PathogenicFoods (Basel, Switzerland) · 2023Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA visual, rapid, simple method was developed based on a loop-mediated isothermal amplification (LAMP) assay to detect Vibrio vulnificus in aquatic products and aquaculture waters.
resultsGenomic DNA was extracted from Vibrio vulnificus using the boiling method, and optimized primers were used to detect the gyrB gene using a visual LAMP method. The sensitivity of the assay was 10 fg/μL, and the obtained results were stable and reliable. Out of 655 aquatic product samples and 558 aquaculture water samples, the positive rates of Vibrio vulnificus detection were 9.01% and 8.60%, respectively, which are markedly higher than those of the traditional culture identification methods.
conclusionThe relatively simple technical requirements, low equipment cost, and rapid detection make the visual LAMP method for the detection of Vibrio vulnificus a convenient choice for field detection in the aquaculture industry.
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