Evidence map›Paper›PMID 36270951›Full record

ArticleNeurology(R) neuroimmunology & neuroinflammation2023

Comparison of Fixed and Live Cell-Based Assay for the Detection of AChR and MuSK Antibodies in Myasthenia Gravis.

Gregorio Spagni, Matteo Gastaldi, Pietro Businaro, Zeineb Chemkhi, Cinzia Carrozza, Giovanni Mascagna, Silvia Falso, Silvia Scaranzin, Diego Franciotta, Amelia Evoli and 1 more

Open access · goldAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Neurology(R) neuroimmunology & neuroinflammation, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed, 1 pooled it
4.7field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Article
  4. Observational
  5. Article
  6. Article
  7. Review
  8. Article
  9. Review
  10. Article
  11. Article
  12. Efgartigimod improves non-AChR generalized Myasthenia Gravis: a real world experience.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2025
    Article
  13. Article
  14. Article
  15. Review
  16. Article
  17. Review
  18. Article
  19. Article
  20. Serological Markers of Clinical Improvement in MuSK Myasthenia Gravis.Neurology(R) neuroimmunology & neuroinflammation · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 2 countries.

Gregorio SpagniFrom the Department of Neuroscience (G.S., Z.C., S.F., A.E., V.D.), Università Cattolica del Sacro Cuore; Fondazione Policlinico Universitario "A. Gemelli" IRCCS (G.S., A.E.), Rome; Neuroimmunology Laboratory (M.G., P.B., S.S.), IRCCS Mondino Foundation; Department of Brain and Behavioral Sciences (P.B.), University of Pavia, Italy; Chemistry, Biochemistry and Clinical Molecular Biology Unit (C.C., G.M.), Fondazione Policlinico "Gemelli," IRCCS, Rome; Autoimmunology Laboratory (D.F.), IRCCS Ospedale Policlinico San Martino, Genoa; and Department of Neurosciences, Drugs and Child Health (V.D.), University of Florence, Italy.
Matteo GastaldiFrom the Department of Neuroscience (G.S., Z.C., S.F., A.E., V.D.), Università Cattolica del Sacro Cuore; Fondazione Policlinico Universitario "A. Gemelli" IRCCS (G.S., A.E.), Rome; Neuroimmunology Laboratory (M.G., P.B., S.S.), IRCCS Mondino Foundation; Department of Brain and Behavioral Sciences (P.B.), University of Pavia, Italy; Chemistry, Biochemistry and Clinical Molecular Biology Unit (C.C., G.M.), Fondazione Policlinico "Gemelli," IRCCS, Rome; Autoimmunology Laboratory (D.F.), IRCCS Ospedale Policlinico San Martino, Genoa; and Department of Neurosciences, Drugs and Child Health (V.D.), University of Florence, Italy.
Pietro BusinaroFrom the Department of Neuroscience (G.S., Z.C., S.F., A.E., V.D.), Università Cattolica del Sacro Cuore; Fondazione Policlinico Universitario "A. Gemelli" IRCCS (G.S., A.E.), Rome; Neuroimmunology Laboratory (M.G., P.B., S.S.), IRCCS Mondino Foundation; Department of Brain and Behavioral Sciences (P.B.), University of Pavia, Italy; Chemistry, Biochemistry and Clinical Molecular Biology Unit (C.C., G.M.), Fondazione Policlinico "Gemelli," IRCCS, Rome; Autoimmunology Laboratory (D.F.), IRCCS Ospedale Policlinico San Martino, Genoa; and Department of Neurosciences, Drugs and Child Health (V.D.), University of Florence, Italy.
Zeineb ChemkhiFrom the Department of Neuroscience (G.S., Z.C., S.F., A.E., V.D.), Università Cattolica del Sacro Cuore; Fondazione Policlinico Universitario "A. Gemelli" IRCCS (G.S., A.E.), Rome; Neuroimmunology Laboratory (M.G., P.B., S.S.), IRCCS Mondino Foundation; Department of Brain and Behavioral Sciences (P.B.), University of Pavia, Italy; Chemistry, Biochemistry and Clinical Molecular Biology Unit (C.C., G.M.), Fondazione Policlinico "Gemelli," IRCCS, Rome; Autoimmunology Laboratory (D.F.), IRCCS Ospedale Policlinico San Martino, Genoa; and Department of Neurosciences, Drugs and Child Health (V.D.), University of Florence, Italy.
Cinzia CarrozzaFrom the Department of Neuroscience (G.S., Z.C., S.F., A.E., V.D.), Università Cattolica del Sacro Cuore; Fondazione Policlinico Universitario "A. Gemelli" IRCCS (G.S., A.E.), Rome; Neuroimmunology Laboratory (M.G., P.B., S.S.), IRCCS Mondino Foundation; Department of Brain and Behavioral Sciences (P.B.), University of Pavia, Italy; Chemistry, Biochemistry and Clinical Molecular Biology Unit (C.C., G.M.), Fondazione Policlinico "Gemelli," IRCCS, Rome; Autoimmunology Laboratory (D.F.), IRCCS Ospedale Policlinico San Martino, Genoa; and Department of Neurosciences, Drugs and Child Health (V.D.), University of Florence, Italy.
Giovanni MascagnaFrom the Department of Neuroscience (G.S., Z.C., S.F., A.E., V.D.), Università Cattolica del Sacro Cuore; Fondazione Policlinico Universitario "A. Gemelli" IRCCS (G.S., A.E.), Rome; Neuroimmunology Laboratory (M.G., P.B., S.S.), IRCCS Mondino Foundation; Department of Brain and Behavioral Sciences (P.B.), University of Pavia, Italy; Chemistry, Biochemistry and Clinical Molecular Biology Unit (C.C., G.M.), Fondazione Policlinico "Gemelli," IRCCS, Rome; Autoimmunology Laboratory (D.F.), IRCCS Ospedale Policlinico San Martino, Genoa; and Department of Neurosciences, Drugs and Child Health (V.D.), University of Florence, Italy.
Silvia FalsoFrom the Department of Neuroscience (G.S., Z.C., S.F., A.E., V.D.), Università Cattolica del Sacro Cuore; Fondazione Policlinico Universitario "A. Gemelli" IRCCS (G.S., A.E.), Rome; Neuroimmunology Laboratory (M.G., P.B., S.S.), IRCCS Mondino Foundation; Department of Brain and Behavioral Sciences (P.B.), University of Pavia, Italy; Chemistry, Biochemistry and Clinical Molecular Biology Unit (C.C., G.M.), Fondazione Policlinico "Gemelli," IRCCS, Rome; Autoimmunology Laboratory (D.F.), IRCCS Ospedale Policlinico San Martino, Genoa; and Department of Neurosciences, Drugs and Child Health (V.D.), University of Florence, Italy.
Silvia ScaranzinFrom the Department of Neuroscience (G.S., Z.C., S.F., A.E., V.D.), Università Cattolica del Sacro Cuore; Fondazione Policlinico Universitario "A. Gemelli" IRCCS (G.S., A.E.), Rome; Neuroimmunology Laboratory (M.G., P.B., S.S.), IRCCS Mondino Foundation; Department of Brain and Behavioral Sciences (P.B.), University of Pavia, Italy; Chemistry, Biochemistry and Clinical Molecular Biology Unit (C.C., G.M.), Fondazione Policlinico "Gemelli," IRCCS, Rome; Autoimmunology Laboratory (D.F.), IRCCS Ospedale Policlinico San Martino, Genoa; and Department of Neurosciences, Drugs and Child Health (V.D.), University of Florence, Italy.
Diego FranciottaFrom the Department of Neuroscience (G.S., Z.C., S.F., A.E., V.D.), Università Cattolica del Sacro Cuore; Fondazione Policlinico Universitario "A. Gemelli" IRCCS (G.S., A.E.), Rome; Neuroimmunology Laboratory (M.G., P.B., S.S.), IRCCS Mondino Foundation; Department of Brain and Behavioral Sciences (P.B.), University of Pavia, Italy; Chemistry, Biochemistry and Clinical Molecular Biology Unit (C.C., G.M.), Fondazione Policlinico "Gemelli," IRCCS, Rome; Autoimmunology Laboratory (D.F.), IRCCS Ospedale Policlinico San Martino, Genoa; and Department of Neurosciences, Drugs and Child Health (V.D.), University of Florence, Italy.
Amelia EvoliFrom the Department of Neuroscience (G.S., Z.C., S.F., A.E., V.D.), Università Cattolica del Sacro Cuore; Fondazione Policlinico Universitario "A. Gemelli" IRCCS (G.S., A.E.), Rome; Neuroimmunology Laboratory (M.G., P.B., S.S.), IRCCS Mondino Foundation; Department of Brain and Behavioral Sciences (P.B.), University of Pavia, Italy; Chemistry, Biochemistry and Clinical Molecular Biology Unit (C.C., G.M.), Fondazione Policlinico "Gemelli," IRCCS, Rome; Autoimmunology Laboratory (D.F.), IRCCS Ospedale Policlinico San Martino, Genoa; and Department of Neurosciences, Drugs and Child Health (V.D.), University of Florence, Italy. amelia.evoli@unicatt.it.
Valentina DamatoFrom the Department of Neuroscience (G.S., Z.C., S.F., A.E., V.D.), Università Cattolica del Sacro Cuore; Fondazione Policlinico Universitario "A. Gemelli" IRCCS (G.S., A.E.), Rome; Neuroimmunology Laboratory (M.G., P.B., S.S.), IRCCS Mondino Foundation; Department of Brain and Behavioral Sciences (P.B.), University of Pavia, Italy; Chemistry, Biochemistry and Clinical Molecular Biology Unit (C.C., G.M.), Fondazione Policlinico "Gemelli," IRCCS, Rome; Autoimmunology Laboratory (D.F.), IRCCS Ospedale Policlinico San Martino, Genoa; and Department of Neurosciences, Drugs and Child Health (V.D.), University of Florence, Italy.
University of Pavia · ITIstituti di Ricovero e Cura a Carattere Scientifico · ITUrology Foundation · GBOspedale Policlinico San Martino · IT

Funding

The Impact of COVID-19 on People Living with Rare Diseases and Their FamiliesU2CTR002818 · NCATS · CINCINNATI CHILDRENS HOSP MED CTR · PI Maurizio Macaluso · 2019 to 2026
$51.2M
Rare Disease Network for Myasthenia GravisU54NS115054 · NINDS · GEORGE WASHINGTON UNIVERSITY · PI Linda Louise Kusner · 2019 to 2026
$11.9M
NCATS NIH HHS U2C TR002818NINDS NIH HHS U54 NS115054
6 · The paper itself

Abstract

BACKGROUND AND

objectivesLive cell-based assay (CBA) can detect acetylcholine receptors (AChRs) or muscle-specific tyrosine kinase (MuSK) antibodies (Abs) in a proportion of patients with radioimmunoassay (RIA)-double seronegative myasthenia gravis (dSN-MG). A commercial fixed CBA for AChR and MuSK Abs has recently become available; however, comparative studies on fixed and live CBAs are lacking. In this study, we compared the performance of fixed and live CBAs in patients with RIA-dSN MG and assessed their sensitivity in RIA-positive MG samples and their specificity.

methodsAChR and MuSK Abs were tested in 292 serum samples from 2 Italian MG referral centers by live and fixed CBAs: 192 from patients with MG and 100 from controls. All samples had been previously assessed by RIA: 66 were AChR positive, 40 MuSK positive, and 86 dSN. All controls were negative. Two independent raters assessed the CBA results. Fixed and live CBAs were compared with the McNemar test; interrater and interlaboratory agreement were assessed with Cohen's kappa or interclass correlation coefficient (ICC), as appropriate.

resultsIn 86 RIA-dSN samples, fixed CBA detected Abs in 10 cases (11.6%, 95% CI 5.7-20.3), whereas live CBA detected Abs in 16 (18.6%, 95% CI 11.0-28.5) ( DISCUSSION: Fixed CBA represents a valuable alternative to RIA for AChR and MuSK Ab detection in patients with MG and could be considered as a first-step diagnostic test. Live CBA can be useful in the serologic evaluation of RIA- and fixed CBA-negative samples.

Indexed as

Myasthenia GravisReceptor Protein-Tyrosine KinasesAutoantibodiesCohort StudiesHumansReceptors, CholinergicAutoantibodiesMUSK protein, humanReceptor Protein-Tyrosine KinasesReceptors, Cholinergic

Identifiers

PMID36270951
PMCPMC9621337
OpenAlexW4307429304

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.