Evidence map›Paper›PMID 36269936›Full record

ArticleNano letters2022

Enabling Spectrally Resolved Single-Molecule Localization Microscopy at High Emitter Densities.

Koen J A Martens, Martijn Gobes, Emmanouil Archontakis, Roger R Brillas, Niels Zijlstra, Lorenzo Albertazzi, Johannes Hohlbein

Abstract read
In one paragraph

Article in Nano letters, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Article
  8. Article
  9. Visualizing the Heterogeneity in Homogeneous Supramolecular Polymers.Journal of the American Chemical Society · 2024
    Article
  10. Review
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Koen J A MartensLaboratory of Biophysics, Wageningen University and Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands.ORCID 0000-0002-9447-8579
Martijn GobesLaboratory of Biophysics, Wageningen University and Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands.
Emmanouil ArchontakisDepartment of Biomedical Engineering, Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology, 5600 MB Eindhoven, Netherlands.
Roger R BrillasDepartment of Biomedical Engineering, Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology, 5600 MB Eindhoven, Netherlands.
Niels ZijlstraLaboratory of Biophysics, Wageningen University and Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands.
Lorenzo AlbertazziDepartment of Biomedical Engineering, Institute for Complex Molecular Systems (ICMS), Eindhoven University of Technology, 5600 MB Eindhoven, Netherlands.ORCID 0000-0002-6837-0812
Johannes HohlbeinLaboratory of Biophysics, Wageningen University and Research, Stippeneng 4, 6708 WE Wageningen, The Netherlands.ORCID 0000-0001-7436-2221

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single-molecule localization microscopy (SMLM) is a powerful super-resolution technique for elucidating structure and dynamics in the life- and material sciences. Simultaneously acquiring spectral information (spectrally resolved SMLM, sSMLM) has been hampered by several challenges: an increased complexity of the optical detection pathway, lower accessible emitter densities, and compromised spatio-spectral resolution. Here we present a single-component, low-cost implementation of sSMLM that addresses these challenges. Using a low-dispersion transmission grating positioned close to the image plane, the +1

Indexed as

MicroscopySingle Molecule ImagingAlgorithmsFluorescent DyesNanotechnologyFluorescent Dyesmulticolor imagingpoint accumulation for imaging in nanoscale topography (PAINT)single-molecule Förster resonance energy transfer (smFRET)Single-molecule spectroscopystochastic optical reconstruction microscopy (STORM)

Identifiers

PMID36269936
PMCPMC9650776

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.