ArticlePloS one2022
In vitro versus cryo-induced capacitation of bovine spermatozoa, part 1: Structural, functional, and oxidative similarities and differences.
Article in PloS one, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.
- A systematic review and meta-analysis of the impact of triclosan exposure on human semen quality.Frontiers in toxicology · 2024Pooled it
- Article
- Bovine In Vitro Fertilization Embryo Development Predicted by Capacitation-Induced Zinc Ion Flux and Acrosome Remodeling.International journal of molecular sciences · 2026Article
- Effects of quercetin supplementation on the quality and metabolomic characteristics of sex-sorted frozen semen from Australian Wagyu bulls.Frontiers in veterinary science · 2026Article
- Sperm dynamics in the female reproductive tract and their role in regulating fertility.The Journal of reproduction and development · 2026Review
- Role of the CaCells · 2025Article
- Type of Extender and Equilibration Time as Factors Affecting Post-Thaw Quality Characteristics of Turkey Semen.Animals : an open access journal from MDPI · 2025Article
- Sperm freezability is associated with melatonin receptor expression in the sperm plasma membrane of Saanen goat bucksVeterinary world · 2025Article
- Integrated Proteomics and Metabolomics Reveal Spermine Enhances Sperm Freezability via Antioxidant Pathways.Antioxidants (Basel, Switzerland) · 2025Article
- Using Cholesterol-Loaded Cyclodextrin to Improve Cryo-Survivability and Reduce Capacitation-Like Changes in Gender-Ablated Jersey Semen.Animals : an open access journal from MDPI · 2025Article
- Techniques to Determine Mammalian Sperm Capacitation.Methods in molecular biology (Clifton, N.J.) · 2025Article
- The Role of Melatonin on Caprine (Antioxidants (Basel, Switzerland) · 2024Review
- Cryoprotective Potential of Theobromine in the Improvement of the Post-Thaw Quality of Bovine Spermatozoa.Cells · 2024Article
- Kaempferol as an Alternative Cryosupplement for Bovine Spermatozoa: Cytoprotective and Membrane-Stabilizing Effects.International journal of molecular sciences · 2024Article
- Article
- The association of resveratrol and AFPI did not enhance the cryoresistance of ram sperm.Animal reproduction · 2024Article
- Profiling of seminal plasma proteins to identify the fertility of Simmental bull with low semen quality.Journal of advanced veterinary and animal research · 2023Article
- Molecular Markers: A New Paradigm in the Prediction of Sperm Freezability.International journal of molecular sciences · 2023Review
- Epicatechin Prevents Cryocapacitation of Bovine Spermatozoa through Antioxidant Activity and Stabilization of Transmembrane Ion Channels.International journal of molecular sciences · 2023Article
- In Vitro versus Cryo-Induced Capacitation of Bovine Spermatozoa, Part 2: Changes in the Expression Patterns of Selected Transmembrane Channels and Protein Kinase A.International journal of molecular sciences · 2022Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Low temperatures during cryopreservation activate a cascade of changes, which may lead into irreversible damage and reduction of the fertilization potential, including the process of premature capacitation. The aim of our study was to evaluate the range of cell damage following the cryopreservation process and possible activation of cryocapacitation in bovine spermatozoa. For the experiments semen samples were obtained from 30 sexually mature Holstein bulls. Within the analysed parameters, we focused on the functional activity, structural integrity, capacitation status and oxidative profile. The samples were divided into three experimental groups, control (CTRL), in vitro capacitated (CAP) and cryopreserved (CRYO). Based on the collected data, there was a significant decrease in the sperm motility, mitochondrial membrane potential and concentration of cyclic adenosine monophosphate in the CRYO group when compared to CAP and CTRL (P<0.0001). A significant decrease (P<0.01; P<0.0001) in the membrane and acrosome integrity as well as DNA fragmentation index and a significant increase (P<0.0001) of necrotic cells were observed in the CRYO group. Following capacitation, a significant increase (P<0.01; P<0.0001) was recorded in the number of cells which underwent the acrosome reaction in the CRYO group against CAP and CTRL. Changes in the oxidative profile of the CRYO group indicates an increase (P<0.0001) in the reactive oxygen species generation, except for the superoxide radical, which was significantly higher (P<0.0001; P<0.001) in the CAP group in comparison with CRYO and CTRL. In summary, premature capacitation may be considered a consequence of cryopreservation and the assessed parameters could serve as physical markers of cryogenic damage to bovine spermatozoa in the future.
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