ArticlePLoS biology2022
GBPL3 localizes to the nuclear pore complex and functionally connects the nuclear basket with the nucleoskeleton in plants.
Article in PLoS biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 40 citations in OpenAlex.
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- Nuclear basket localized proteasomes maintain circadian period through nuclear TOC1 proteolysis.bioRxiv : the preprint server for biology · 2026Article
- Nuclear Entanglement: New Insights Into the Role of Cytoskeleton and Nucleoskeleton in Plant Nuclear Function.Cytoskeleton (Hoboken, N.J.) · 2026Review
- A multiomics atlas of theScience advances · 2026Article
- SUMO E3 ligase SIZ1 promotes nuclear condensate-mediated immune activation in Arabidopsis.Nature communications · 2026Article
- The role of phase separation for RNA and protein transport through the nuclear pore complex.Journal of experimental botany · 2026Review
- Leaf it to science: Uncovering plant immune systems through technological advances.Plant physiology · 2026Review
- Guanylate-binding proteins: emerging critical functions in plant development, immunity and stress response.The New phytologist · 2025Review
- In situ architecture of the nuclear pore complex of the higher plant Arabidopsis thaliana.Nature plants · 2025Article
- A nucleoporin-associated signaling cascade controls plant immunity via histone modification.Genome biology · 2025Article
- Living with temperature changes: Salicylic acid at the crossroads of plant immunity and temperature resilience.Science advances · 2025Review
- Biomolecular condensates in plant immunity.Cell host & microbe · 2025Review
- The Nucleoporin CPR5 Modulates Plant Immunity via Guanylate-Binding Proteins.Molecular plant pathology · 2025Article
- Nucleoporin PNET1 coordinates mitotic nuclear pore complex dynamics for rapid cell division.Nature plants · 2025Article
- Unraveling Plant Nuclear Envelope Composition Using Proximity Labeling Proteomics.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Next-generation mapping of the salicylic acid signaling hub and transcriptional cascade.Molecular plant · 2024Article
- CRWN nuclear lamina components maintain the H3K27me3 landscape and promote successful reproduction in Arabidopsis.The New phytologist · 2024Article
- Proxiome assembly of the plant nuclear pore reveals an essential hub for gene expression regulation.Nature plants · 2024Article
- Salicylic acid in plant immunity and beyond.The Plant cell · 2024Review
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nuclear basket (NB) is an essential structure of the nuclear pore complex (NPC) and serves as a dynamic and multifunctional platform that participates in various critical nuclear processes, including cargo transport, molecular docking, and gene expression regulation. However, the underlying molecular mechanisms are not completely understood, particularly in plants. Here, we identified a guanylate-binding protein (GBP)-like GTPase (GBPL3) as a novel NPC basket component in Arabidopsis. Using fluorescence and immunoelectron microscopy, we found that GBPL3 localizes to the nuclear rim and is enriched in the nuclear pore. Proximity labeling proteomics and protein-protein interaction assays revealed that GBPL3 is predominantly distributed at the NPC basket, where it physically associates with NB nucleoporins and recruits chromatin remodelers, transcription apparatus and regulators, and the RNA splicing and processing machinery, suggesting a conserved function of the NB in transcription regulation as reported in yeasts and animals. Moreover, we found that GBPL3 physically interacts with the nucleoskeleton via disordered coiled-coil regions. Simultaneous loss of GBPL3 and one of the 4 Arabidopsis nucleoskeleton genes CRWNs led to distinct development- and stress-related phenotypes, ranging from seedling lethality to lesion development, and aberrant transcription of stress-related genes. Our results indicate that GBPL3 is a bona fide component of the plant NPC and physically and functionally connects the NB with the nucleoskeleton, which is required for the coordination of gene expression during plant development and stress responses.
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