ArticleJournal of pathology informatics2022
The impact of different coverslipping methods in the quality of the whole slide images used for diagnosis in pathology.
Article in Journal of pathology informatics, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
8 citing papers in PubMed, 16 citations in OpenAlex.
- Whole-slide imaging in hematopathology: Current state, challenges and future opportunities.Journal of pathology informatics · 2026Review
- Decoverslipping of Archival Cytology Aspirate Smears for Ancillary Studies: A Review of Methods and Institutional Experience With Freezer-Based Techniques.Cytopathology : official journal of the British Society for Clinical Cytology · 2026Review
- GrandQC adaptation as an artificial intelligence tool for quantitative artifact detection in hematoxylin and eosin whole-slide images-Simulation of quality control biopsies day.Journal of pathology informatics · 2026Article
- Digital transformation of pathology - the European Society of Pathology expert opinion paper.Virchows Archiv : an international journal of pathology · 2025Article
- Optimizing the management of thyroid specimens to efficiently generate whole slide images for diagnosis.Virchows Archiv : an international journal of pathology · 2024Article
- Immunolabelling perturbs the endogenous and antibody-conjugated elemental concentrations during immuno-mass spectrometry imaging.Analytical and bioanalytical chemistry · 2024Article
- Cutting-edge technology and automation in the pathology laboratory.Virchows Archiv : an international journal of pathology · 2024Review
- [Effects of upstream laboratory processes on the digitization of histological slides].Pathologie (Heidelberg, Germany) · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Digital pathology workflow aims to create whole slide images (WSIs) for diagnosis. The quality of the WSIs depends primarily on the quality of the glass slides produced by the pathology laboratory, where the coverslipping method plays an important role. In this study we compare the glass, the film, and the liquid coverslipping methods to evaluate which ones are suitable to create WSIs for diagnosis. The study included 18 formalin-fixed paraffin-embedded tissue blocks. Of each block, 3 consecutive sections were covered using 1 of the 3 methods. The slides were scanned and evaluated for quality criteria by 2 pathologists experienced in digital pathology. The coverslipping method interferes with the quality of the WSIs, as well as with the scanning time and the file size of the WSIs. All coverslipping methods were found suitable for diagnosis. The glass and liquid methods were manual and had similar results concerning the presence of air bubbles/polymer accumulation, air drying artefacts, tissue exposed, and staining alterations. The glass method was the one with more air bubbles. The liquid method was associated with more alterations on the WSIs, but with the lowest file sizes. Automation of coverslipping and calibration of the scanner for the coverslipping method chosen by the pathology laboratory are relevant for the final quality of the WSIs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.