ArticleCommunications biology2022
Expression of divergent methyl/alkyl coenzyme M reductases from uncultured archaea.
Article in Communications biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 25 citations in OpenAlex.
- Atomic resolution structures of the methane-activating enzyme in anaerobic methanotrophy reveal extensive post-translational modifications.Nature communications · 2025Article
- Industrial applicability of enzymatic and whole-cell processes for the utilization of C1 building blocks.Nature communications · 2025Review
- Identification of key steps in the evolution of anaerobic methanotrophy inScience advances · 2025Article
- Article
- The crystal structure of methanogen McrD, a methyl-coenzyme M reductase-associated protein.FEBS open bio · 2024Article
- Genomic language model predicts protein co-regulation and function.Nature communications · 2024Article
- Assessing the Natural Source Zone Depletion of a Petroleum-Contaminated Clayey Soil Site in Southern China Combining Concentration Gradient Method and Metagenomics.Life (Basel, Switzerland) · 2024Article
- Editorial: Cross-boundary significance of methanogens - the methane moment and beyond.Frontiers in microbiology · 2024Article
- Metagenomic profiles of archaea and bacteria within thermal and geochemical gradients of the Guaymas Basin deep subsurface.Nature communications · 2023Article
- Respiration-driven methanotrophic growth of diverse marine methanogens.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- McrD binds asymmetrically to methyl-coenzyme M reductase improving active-site accessibility during assembly.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The methanogen core and pangenome: conservation and variability across biology's growth temperature extremes.DNA research : an international journal for rapid publication of reports on genes and genomes · 2023Article
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
Methanogens and anaerobic methane-oxidizing archaea (ANME) are important players in the global carbon cycle. Methyl-coenzyme M reductase (MCR) is a key enzyme in methane metabolism, catalyzing the last step in methanogenesis and the first step in anaerobic methane oxidation. Divergent mcr and mcr-like genes have recently been identified in uncultured archaeal lineages. However, the assembly and biochemistry of MCRs from uncultured archaea remain largely unknown. Here we present an approach to study MCRs from uncultured archaea by heterologous expression in a methanogen, Methanococcus maripaludis. Promoter, operon structure, and temperature were important determinants for MCR production. Both recombinant methanococcal and ANME-2 MCR assembled with the host MCR forming hybrid complexes, whereas tested ANME-1 MCR and ethyl-coenzyme M reductase only formed homogenous complexes. Together with structural modeling, this suggests that ANME-2 and methanogen MCRs are structurally similar and their reaction directions are likely regulated by thermodynamics rather than intrinsic structural differences.
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