ArticleNature communications2022
Cryogenic contrast-enhanced microCT enables nondestructive 3D quantitative histopathology of soft biological tissues.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 46 citations in OpenAlex.
- Loss ofiScience · 2026Article
- Stepwise micro-CT analysis of sample processing effects on the 3D morphology of human intervertebral discs.Osteoarthritis and cartilage open · 2026Article
- Bioimaging of sense organs and the central nervous system in extant fishes and reptiles in situ: A review.Anatomical record (Hoboken, N.J. : 2007) · 2026Review
- Sodium-myo-inositol cotransporter-1, SMIT1, promotes cardiac hypertrophy and fibrosis induced by pressure overload in mice.Cardiovascular research · 2026Article
- A lung CT vision foundation model facilitating disease diagnosis and medical imaging.Nature communications · 2025Article
- Evaluating the impact of contrast agents on micro and nano mechanics of soft-to-hard tissue interface.Scientific reports · 2025Article
- Fine visualization of biological cells using X-ray micro-CT with the slow freezing contrast improved method.Scientific reports · 2025Article
- MicroCT and contrast-enhanced microCT to study the in vivo degradation behavior and biocompatibility of candidate metallic intravascular stent materials.Acta biomaterialia · 2025Article
- Cryo-X-Ray Phase Contrast Imaging Enables Combined 3D Structural Quantification and Nucleic Acid Analysis of Myocardial Biopsies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- 2D and 3D microstructural analysis of the iliotibial tract.Journal of anatomy · 2024Article
- Synchrotron X-ray imaging of soft biological tissues - principles, applications and future prospects.Journal of cell science · 2024Review
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- X-Ray-Based 3D Histopathology of the Kidney Using Cryogenic Contrast-Enhanced MicroCT.International journal of biomedical imaging · 2024Article
- Collagen molecular organization preservation in human fascia lata and periosteum after tissue engineering.Frontiers in bioengineering and biotechnology · 2024Article
- Col1A-2 Mutation in Osteogenesis Imperfecta Mice Contributes to Long Bone Fragility by Modifying Cell-Matrix Organization.International journal of molecular sciences · 2023Article
- Gold nanoparticle-enhanced X-ray microtomography of the rodent reveals region-specific cerebrospinal fluid circulation in the brain.Nature communications · 2023Article
- An optically-guided cochlear implant sheath for real-time monitoring of electrode insertion into the human cochlea.Scientific reports · 2022Article
- Cryogenic contrast-enhanced microCT enables nondestructive 3D quantitative histopathology of soft biological tissues.Nature communications · 2022Article
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biological tissues comprise a spatially complex structure, composition and organization at the microscale, named the microstructure. Given the close structure-function relationships in tissues, structural characterization is essential to fully understand the functioning of healthy and pathological tissues, as well as the impact of possible treatments. Here, we present a nondestructive imaging approach to perform quantitative 3D histo(patho)logy of biological tissues, termed Cryogenic Contrast-Enhanced MicroCT (cryo-CECT). By combining sample staining, using an X-ray contrast-enhancing staining agent, with freezing the sample at the optimal freezing rate, cryo-CECT enables 3D visualization and structural analysis of individual tissue constituents, such as muscle and collagen fibers. We applied cryo-CECT on murine hearts subjected to pressure overload following transverse aortic constriction surgery. Cryo-CECT allowed to analyze, in an unprecedented manner, the orientation and diameter of the individual muscle fibers in the entire heart, as well as the 3D localization of fibrotic regions within the myocardial layers. We foresee further applications of cryo-CECT in the optimization of tissue/food preservation and donor banking, showing that cryo-CECT also has clinical and industrial potential.
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