Evidence map›Paper›PMID 36264513›Full record

ReviewArchives of microbiology2022

Methods used for the study of endophytic fungi: a review on methodologies and challenges, and associated tips.

Jefferson Brendon Almeida Dos Reis, Adriana Sturion Lorenzi, Helson Mario Martins do Vale

Open access · bronzeAbstract readReview
In one paragraph

Review in Archives of microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.

0numbers the graph read from it
0cells of the map it votes in
50citing papers in PubMed
11.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

50 citing papers in PubMed, 112 citations in OpenAlex.

  1. Article
  2. Endophytic Fungi from WildJournal of fungi (Basel, Switzerland) · 2026
    Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Chemical and Biological Investigation of the EndophyticJournal of fungi (Basel, Switzerland) · 2026
    Article
  11. Novel Endophytic Fungi fromMicroorganisms · 2026
    Article
  12. Review
  13. Review
  14. Identification and Multigene Phylogenetic Analysis RevealJournal of fungi (Basel, Switzerland) · 2026
    Article
  15. Review
  16. Review
  17. Article
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 2 countries.

Jefferson Brendon Almeida Dos ReisDepartment of Phytopathology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, DF, Brazil. jeffersonalmeidareis@gmail.com.ORCID http://orcid.org/0000-0002-2768-214X
Adriana Sturion LorenziDepartment of Cellular Biology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, DF, Brazil.
Helson Mario Martins do ValeDepartment of Phytopathology, Institute of Biological Sciences, University of Brasília (UnB), Brasília, DF, Brazil.
Universidade de Brasília · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Endophytic fungi are microorganisms that colonize the interior of plant tissues (e.g. leaves, seeds, stem, trunk, roots, fruits, flowers) in intracellular and/or extracellular spaces without causing symptoms of disease in host plants. These microorganisms have been isolated from plant species in a wide variety of habitats worldwide, and it is estimated that all terrestrial plants are colonized by one or more species of endophytic fungus. In addition, these microorganisms have been drawing the attention of researchers because of their ability to synthesize a wide range of bioactive molecules with potential for applications in agriculture, medicine and biotechnology. However, several obstacles come up when studying the diversity and chemical potential of endophytic fungi. For example, the usage of an inappropriate surface disinfection method for plant tissue may not eliminate the epiphytic microbiota or may end up interfering with the endophytic mycobiota, which consequently generates erroneous results. Moreover, the composition of the culture medium and the culture conditions can favor the growth of certain species and inhibit others, which generates underestimated results. Other inconsistencies can arise from the fungus misidentification and consequent exploration of its chemical potential. Based on the methodological biases that may occur at all stages of studies dealing with endophytic fungi, the objective of this review is to discuss the main methods employed in these studies as well as highlight the challenges derived from the different approaches. We also report associated tips to help future studies on endophytic fungi as a contribution.

Indexed as

EndophytesFungiPlant LeavesPlant RootsPlantsDiversityEndophyteITSMolecular identificationMycobiomeSymbiosis

Identifiers

PMID36264513
PMCPMC9584250
OpenAlexW4306901869

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.