ArticleParasites & vectors2022
First genetic report of Ixodes kashmiricus and associated Rickettsia sp.
Article in Parasites & vectors, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 25 citations in OpenAlex.
- First Molecular Report of Haemaphysalis shimoga and Haemaphysalis bispinosa Infesting Captive Sambar Deer (Rusa unicolor) in Malaysia, with Detection of Anaplasma phagocytophilum and Theileria Co-infection.Acta parasitologica · 2026Article
- Genetic diversity and co-circulation of tick-borne pathogens in Haemaphysalis spp. from Pakistan.Parasitology research · 2026Article
- Genetic and morphological data indicate the occurrence of a Rhipicephalus sp. and the absence of Rhipicephalus linnaei in Pakistan.Tropical animal health and production · 2026Article
- Molecular-based detection of Ehrlichia spp. in stray dogs-infesting Rhipicephalus ticks in high-altitude of northern Pakistan.PloS one · 2026Article
- Molecular Profiling of Ticks and Associated Pathogens: First Report ofTransboundary and emerging diseases · 2025Article
- Molecular detection of Anaplasma bovis, Candidatus Anaplasma boleense and Rickettsia spp. in ticks infesting small ruminants.BMC veterinary research · 2024Article
- The influence of forest habitat type onCurrent research in parasitology & vector-borne diseases · 2024Article
- Genotyping of ticks: first molecular report ofFrontiers in cellular and infection microbiology · 2024Article
- Checklist of hosts, illustrated geographical range, and ecology of tick species from the genusZooKeys · 2024Article
- Molecular Survey ofVeterinary sciences · 2023Article
- Article
- First Detection ofAnimals : an open access journal from MDPI · 2023Article
- Genetic analysis challenges the presence of Ixodes inopinatus in Central Europe: development of a multiplex PCR to distinguish I. inopinatus from I. ricinus.Parasites & vectors · 2023Article
- Molecular Detection ofVeterinary sciences · 2023Article
- Association of SFGPathogens (Basel, Switzerland) · 2023Article
- First Report ofAnimals : an open access journal from MDPI · 2023Article
- Phylogenetic Position ofGenes · 2023Article
- First report on tick-borne pathogens detected in ticks infesting stray dogs near butcher shops.Frontiers in veterinary science · 2023Article
- First report of spotted fever groupFrontiers in microbiology · 2023Article
- Molecular detection ofFrontiers in microbiology · 2023Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHard ticks (Ixodidae) are hematophagous ectoparasites that transmit various pathogens to a variety of hosts including humans. Transhumant herds have been involved in the spread of ticks and associated Rickettsia spp., and studies on this neglected topic have been unexplored in many regions including Pakistan. This study aimed to investigate ticks infesting transhumant herds of sheep (Ovis aries) and goats (Capra hircus) in district Shangla, Khyber Pakhtunkhwa, Pakistan.
methodsOf the 144 examined animals, 112 hosts (68 sheep and 44 goats) of transhumant herds were infested by 419 ticks of different life stages including nymphs (105; 25%), males (58; 14%) and females (256; 61%). For molecular analyses, DNA was extracted from 64 collected ticks and subjected to PCR for the amplification of tick 16S rDNA and ITS2 partial sequences and for the amplification of rickettsial gltA and ompA gene sequences.
resultsAll tick specimens were identified as Ixodes kashmiricus based on morphological features. The obtained 16S rDNA and ITS2 sequences showed 95.7% and 95.3% identity, respectively, with Ixodes kazakstani reported from Kyrgyzstan. In the phylogenetic tree, the sequences clustered with members of the Ixodes ricinus species complex, including I. kazakstani and Ixodes apronophorus. Additionally, rickettsial gltA and ompA partial sequences were 99.7% identical to Rickettsia sp. endosymbiont of Ixodes spp. from Panama and Costa Rica and 99.2% with Rickettsia endosymbiont from the USA. Phylogenetically, the rickettsial gltA and ompA partial sequences from I. kashmiricus clustered with various haplotypes of Rickettsia endosymbiont, which were sister cladded to Rickettsia monacensis.
conclusionsThis is the first genetic report of I. kashmiricus and associated Rickettsia sp. Large-scale tick surveillance studies across the country are needed to investigate Ixodes ticks and associated pathogens.
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