Evidence map›Paper›PMID 36260988›Full record

ArticleCell reports. Medicine2022

In vitro germ cell induction from fertile and infertile monozygotic twin research participants.

Erica C Pandolfi, Fei-Man Hsu, Mark Duhon, Yi Zheng, Sierra Goldsmith, Jianping Fu, Sherman J Silber, Amander T Clark

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports. Medicine, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. In Vitro Maturation, In Vitro Oogenesis, and Ovarian Longevity.Reproductive sciences (Thousand Oaks, Calif.) · 2024
    Review
  3. Article
  4. Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Erica C PandolfiDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, USA.
Fei-Man HsuDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, USA.
Mark DuhonEli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA, USA.
Yi ZhengDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI, USA.
Sierra GoldsmithInfertility Center of St. Louis, St. Luke's Hospital, St. Louis, MO, USA.
Jianping FuDepartment of Mechanical Engineering, University of Michigan, Ann Arbor, MI, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA; Department of Cell & Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, USA.
Sherman J SilberInfertility Center of St. Louis, St. Luke's Hospital, St. Louis, MO, USA.
Amander T ClarkDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, USA; Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, USA. Electronic address: clarka@ucla.edu.
University of California, Los Angeles · USSt. Luke's Hospital · USUniversity of Michigan · US

Funding

UPLIFT: UCLA Postdocs' Longitudinal Investment in Faculty TrainingK12GM106996 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CAREY, MICHAEL F, VILLANUEVA, CLAUDIO J · 2016 to 2025
$7.1M
Studying Origin of Human Primordial Germ Cells Using a Synthetic Stem Cell ModelR21HD100931 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI FU, JIANPING · 2020 to 2021
$422k
Restoring Germline Potential to Patients Diagnosed with Primary Ovarian InsufficiencyF32HD105439 · NICHD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI PANDOLFI, ERICA CHRISTINE · 2021 to 2021
$36k
Bill & Melinda Gates Foundation INV-003385NICHD NIH HHS F32 HD105439NICHD NIH HHS R21 HD100931NIGMS NIH HHS K12 GM106996
6 · The paper itself

Abstract

Human induced pluripotent stem cells (hiPSCs) enable reproductive diseases to be studied when the reproductive health of the participant is known. In this study, monozygotic (MZ) monoamniotic (MA) twins discordant for primary ovarian insufficiency (POI) consent to research to address the hypothesis that discordant POI is due to a shared primordial germ cell (PGC) progenitor pool. If this is the case, reprogramming the twin's skin cells to hiPSCs is expected to restore equivalent germ cell competency to the twins hiPSCs. Following reprogramming, the infertile MA twin's cells are capable of generating human PGC-like cells (hPGCLCs) and amniotic sac-like structures equivalent to her fertile twin sister. Using these hiPSCs together with genome sequencing, our data suggest that POI in the infertile twin is not due to a genetic barrier to amnion or germ cell formation and support the hypothesis that during gestation, amniotic PGCs are likely disproportionately allocated to the fertile twin with embryo splitting.

Indexed as

Induced Pluripotent Stem CellsInfertilityAmnionEmbryo, MammalianFemaleGerm CellsHumansTwins, Monozygoticamnion cellsdiscordant POIembryo modelgerm cellshiPSCstwins

Identifiers

PMID36260988
PMCPMC9589117
OpenAlexW4306747639

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.