Evidence map›Paper›PMID 36260151›Full record

ArticleJournal of molecular histology2022

Insm1 promotes differentiation of retinal progenitor cells toward photoreceptor cells in the developing retina through up-regulation of SHH.

Pengyi Zhou, Youmei Xu, Lin Yang, Pan Meng, Ju Guo, Zhenhui Liu, Bo Jin, Kunpeng Xie, Haiyan Zhu, Liping Du and 1 more

Abstract read
PubMed Publisher
In one paragraph

Article in Journal of molecular histology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 89% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Pengyi ZhouDepartment of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe East Rd, 450052, Zhengzhou, Henan Province, People's Republic of China. pengy_zhou@163.com.
Youmei XuDepartment of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe East Rd, 450052, Zhengzhou, Henan Province, People's Republic of China.
Lin YangDepartment of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe East Rd, 450052, Zhengzhou, Henan Province, People's Republic of China.
Pan MengDepartment of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe East Rd, 450052, Zhengzhou, Henan Province, People's Republic of China.
Ju GuoDepartment of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe East Rd, 450052, Zhengzhou, Henan Province, People's Republic of China.
Zhenhui LiuDepartment of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe East Rd, 450052, Zhengzhou, Henan Province, People's Republic of China.
Bo JinDepartment of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe East Rd, 450052, Zhengzhou, Henan Province, People's Republic of China.
Kunpeng XieDepartment of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe East Rd, 450052, Zhengzhou, Henan Province, People's Republic of China.
Haiyan ZhuDepartment of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe East Rd, 450052, Zhengzhou, Henan Province, People's Republic of China.
Liping DuDepartment of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe East Rd, 450052, Zhengzhou, Henan Province, People's Republic of China.
Xuemin JinDepartment of Ophthalmology, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe East Rd, 450052, Zhengzhou, Henan Province, People's Republic of China. xuemin_jin@163.com.
First Affiliated Hospital of Zhengzhou University · CN

Funding

Medical Science and Technology Project of Health Commission of Henan Province No.YXKC2020026Medical Science and Technology Research Program of Henan Province No.201602081National Natural Science Foundation of China No.81770914, No.81970792, No.8217040571 and No.81800832
6 · The paper itself

Abstract

This article investigated the effect of Insm1 on RPC differentiation in mice and the underlying mechanism. The retinal tissues of mouse embryo at 12.5 days (E12.5) and postnatal 14 days (P14) were collected, following by the detection of Insm1 and corresponding markers by immunofluorescent staining. RPCs isolated from retinal tissues at P1 were cultured in culture medium for 7 days. The differentiation of photoreceptor and glial cells was assessed after RPCs transferred to the differentiation medium for 20 days. Next, the effect of Insm1 overexpression on the differentiation of RPCs toward rod photoreceptor and glial cells were assessed. Insm1 was highly expressed in RPCs of retinal tissues and decline in photoreceptor cells, while hardly expressed in glial cells. Based on the results of Pax-6 positive immunofluorescent staining and flow cytometry detection, RPCs were successfully isolated from retinal tissues. After the culture in differentiation medium, RPCs showed positive staining of Rhodopsin and glial fibrillary acidic protein (GFAP). Further results showed that overexpression of Insm1 significantly increased the percentage of Rhodopsin positive cells, and up-regulated Sonic Hedgehog (SHH), hairy and enhancer of split homolog-1(Hes1), S-opsin and Rhodopsin levels, while decreased the percentage of Glutamine synthetase positive cells, and reduced Glutamine synthetase and GFAP levels. Whereas, the effect of Insm1 overexpression on these protein levels were partly abolished by the knockdown of SHH or Hes1. We conclude that Insm1 promotes the differentiation of RPCs into photoreceptor cells in the developing retina through up-regulation of SHH.

Indexed as

Glutamate-Ammonia LigaseRhodopsinAnimalsCell DifferentiationHedgehog ProteinsMicePhotoreceptor CellsRepressor ProteinsRetinaStem CellsUp-RegulationGlutamate-Ammonia LigaseHedgehog ProteinsInsm1 protein, mouseRepressor ProteinsRhodopsinShh protein, mouseHes1Insm1Retinal progenitor cell (RPC) differentiationRod photoreceptor cellsSHH

Identifiers

PMID36260151
OpenAlexW4306776519

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.