Evidence map›Paper›PMID 36260075›Full record

ArticleJournal of medical entomology2023

Establishment of a Culex tarsalis (Diptera: Culicidae) Cell Line and its Permissiveness to Arbovirus Infection.

Erin E Schirtzinger, Dane C Jasperson, Dustin A Swanson, Dana Mitzel, Barbara S Drolet, Juergen A Richt, William C Wilson

Abstract read
In one paragraph

Article in Journal of medical entomology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Erin E SchirtzingerArthropod-borne Animal Diseases Research Unit, USDA, ARS, Manhattan, KS, USA.
Dane C JaspersonArthropod-borne Animal Diseases Research Unit, USDA, ARS, Manhattan, KS, USA.
Dustin A SwansonArthropod-borne Animal Diseases Research Unit, USDA, ARS, Manhattan, KS, USA.
Dana MitzelForeign Arthropod-borne Animal Diseases Research Unit, National Bio- and Agro-Defense Facility, USDA, ARS, Manhattan, KS, USA.
Barbara S DroletArthropod-borne Animal Diseases Research Unit, USDA, ARS, Manhattan, KS, USA.
Juergen A RichtDepartment of Diagnostic Medicine/Pathology, College of Veterinary Medicine, Kansas State University, Manhattan, KS, USA.
William C WilsonForeign Arthropod-borne Animal Diseases Research Unit, National Bio- and Agro-Defense Facility, USDA, ARS, Manhattan, KS, USA.ORCID 0000-0002-7877-9224

Funding

NIAID Centers of Excellence for Influenza Research and Response: Universal Influenza Vaccine Research Activities75N93021C00016 · NIAID · ST. JUDE CHILDREN'S RESEARCH HOSPITAL · PI WEBBY, RICHARD · 2021 to 2025
$91.4M
Sex-specific effects of obestity on Influenza A virus infection and immunityP20GM130448 · NIGMS · KANSAS STATE UNIVERSITY · PI REVATHI GOVIND · 2020 to 2026
$15.8M
NIAID NIH HHS 75N93021C00016NIGMS NIH HHS P20 GM130448NIH HHS P20GM130448
6 · The paper itself

Abstract

A cell line was established from Culex tarsalis Coquillett embryonated eggs and designated as CxTr. The cell line is heterogeneous, composed predominantly of small, round cells, and spindle-shaped cells with a doubling time of approximately 52-60 h. The identity of the cell line was verified as Cx. tarsalis by sequencing of cytochrome oxidase I and the cells were found to be free of contaminating cells, bacteria, fungi, and mycoplasma. The permissiveness of CxTr cells to arbovirus infection was investigated with vaccine and wildtype arboviruses from four viral families: Flaviviridae (Japanese encephalitis virus), Phenuiviridae (Rift Valley fever phlebovirus), Rhabdoviridae (vesicular stomatitis virus), and Togaviridae (Mayaro virus). All viruses were able to infect and replicate within CxTr cells.

Indexed as

Arbovirus InfectionsCulexCulicidaeAnimalsCell LinePermissivenessarboviruscell lineCulex tarsalismosquito

Identifiers

PMID36260075
PMCPMC10091495

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.