Evidence map›Paper›PMID 36259950›Full record

ArticleBrain and behavior2022

Structural brain abnormalities in endothelial nitric oxide synthase-deficient mice revealed by high-resolution magnetic resonance imaging.

Hannah George, Grace V Mercer, Darcie Stapleton, Laura Dawson, Phillip E MacCallum, Shoshana Spring, John G Sled, Jacqueline Blundell, Lindsay S Cahill

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brain and behavior, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Cerebrovascular Endothelial Dysfunction: Role of BACE1.Arteriosclerosis, thrombosis, and vascular biology · 2024
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Hannah GeorgeDepartment of Chemistry, Memorial University of Newfoundland, St. John's, Canada.
Grace V MercerDepartment of Chemistry, Memorial University of Newfoundland, St. John's, Canada.
Darcie StapletonDepartment of Chemistry, Memorial University of Newfoundland, St. John's, Canada.
Laura DawsonDepartment of Psychology, Memorial University of Newfoundland, St. John's, Canada.
Phillip E MacCallumDepartment of Psychology, Memorial University of Newfoundland, St. John's, Canada.
Shoshana SpringMouse Imaging Centre, Hospital for Sick Children, Toronto, Canada.
John G SledMouse Imaging Centre, Hospital for Sick Children, Toronto, Canada.
Jacqueline BlundellDepartment of Psychology, Memorial University of Newfoundland, St. John's, Canada.
Lindsay S CahillDepartment of Chemistry, Memorial University of Newfoundland, St. John's, Canada.ORCID 0000-0003-1667-8564
Memorial University of Newfoundland · CAHospital for Sick Children · CAUniversity of Toronto · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionEndothelial nitric oxide synthase (eNOS) produces nitric oxide, which is essential for a variety of physiological functions in the brain. Previous work has demonstrated the detrimental effects of eNOS deficiency on brain function in male eNOS knockout (eNOS KO) mice. However, the effect of eNOS deficiency on brain structure and any association between these effects and sex is unknown.

methodsThis study used three-dimensional high-resolution ex vivo magnetic resonance imaging and behavioral tests of anxiety and cognitive performance to investigate structure-function relationships in the brain of female and male eNOS KO mice in young adulthood.

resultsWhile there were no differences in anxiety-like behavior or locomotion, there was a sex-specific deficit in contextual fear memory retention in male, but not in female, eNOS mice compared to wild-type controls. Moreover, we found that eNOS deficiency induced changes in multiple brain regions that are involved in learning and fear memory including the hippocampus, amygdala, hypothalamus, and areas of the cortex. Several of these MRI-detectable neuroanatomical changes were dependent on sex.

conclusionThe observation that eNOS deficiency impacts brain structure at an early age demonstrates the importance of eNOS for healthy brain development.

Indexed as

BrainNitric Oxide Synthase Type IIIAnimalsFemaleMagnetic Resonance ImagingMaleMiceMice, Inbred C57BLMice, KnockoutNitric OxideNitric OxideNitric Oxide Synthase Type IIINos3 protein, mouseendothelial nitric oxide synthasemagnetic resonance imagingmousesex differences

Identifiers

PMID36259950
PMCPMC9660425
OpenAlexW4306793126

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.