Evidence map›Paper›PMID 36259144›Full record

ArticleMicrobiology and immunology2023

Demonstration of bacterium-free very rapid reverse genetics system using mumps virus.

Chaewon Bae, Hiroshi Katoh, Aika Wakata, Masafumi Sakata, Fumihiro Kato, Makoto Takeda

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology and immunology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Chaewon BaeDepartment of Virology III, National Institute of Infectious Diseases, Tokyo, Japan.
Hiroshi KatohDepartment of Virology III, National Institute of Infectious Diseases, Tokyo, Japan.ORCID http://orcid.org/0000-0002-8613-4717
Aika WakataDepartment of Virology III, National Institute of Infectious Diseases, Tokyo, Japan.
Masafumi SakataDepartment of Virology III, National Institute of Infectious Diseases, Tokyo, Japan.
Fumihiro KatoDepartment of Virology III, National Institute of Infectious Diseases, Tokyo, Japan.ORCID http://orcid.org/0000-0002-4547-6905
Makoto TakedaDepartment of Virology III, National Institute of Infectious Diseases, Tokyo, Japan.ORCID http://orcid.org/0000-0002-8194-7727

Funding

Japan Agency for Medical Research and Development JP21fk0108087j0403Japan Agency for Medical Research and Development JP21fk0108617j0201Japan Agency for Medical Research and Development JP21fk0108623j0001Japan Agency for Medical Research and Development JP21wm0325024j0002Japan Society for the Promotion of Science 19K07584Japan Society for the Promotion of Science 22K07108
6 · The paper itself

Abstract

The reverse genetics system is a very powerful tool for analyzing the molecular mechanisms of viral propagation and pathogenesis. However, full-length genome plasmid construction is highly time-consuming and laborious, and undesired mutations may be introduced by Escherichia coli. This study shows a very rapid E. coli-free method of full-genome construction using the mumps virus as an example. This method was able to reduce dramatically the time for full-genome construction, which was used very efficiently for virus rescue, from several days or more to ~2 days, with a similar accuracy and yield to the conventional method using E. coli/plasmid.

Indexed as

Mumps virusReverse GeneticsCloning, MolecularEscherichia coliGenes, ViralGenome, ViralPlasmidsEscherichia coli-freemumps virusOriCiro cell-free cloning systemPCRreverse genetics

Identifiers

PMID36259144
PMCPMC10092663

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.