Evidence map›Paper›PMID 36258720›Full record

ArticleIndian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion2022

High-resolution Melting Analysis for NOTCH1 c.7541-7542delCT Mutation in Chronic Lymphocytic Leukemia: Prognostic Significance in Egyptian Patients.

Dalia Sherief, Asmaa Hassan, Eman Habeeb, Nahla Nosair, Maaly Mabrouk, Sarah Shoeib, Heba Reyad, Tamer Haydra, Mona Watany

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In one paragraph

Article in Indian journal of hematology & blood transfusion : an official journal of Indian Society of Hematology and Blood Transfusion, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 96% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Dalia SheriefClinical Pathology Department, Faculty of Medicine, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt.ORCID 0000-0003-1046-643X
Asmaa HassanClinical Pathology Department, Faculty of Medicine, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt.
Eman HabeebClinical Pathology Department, Faculty of Medicine, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt.
Nahla NosairClinical Pathology Department, Faculty of Medicine, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt.
Maaly MabroukClinical Pathology Department, Faculty of Medicine, Tanta University, Tanta, Egypt.
Sarah ShoeibClinical Pathology Department, Faculty of Medicine, Tanta University, Tanta, Egypt.
Heba ReyadDepartment of Pediatrics, Faculty of Medicine, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt.
Tamer HaydraInternal Medicine Department, Faculty of Medicine, Kafr El-Sheikh University, Kafr El-Sheikh, Egypt.
Mona WatanyClinical Pathology Department, Faculty of Medicine, Tanta University, Tanta, Egypt.
Kafrelsheikh University · EGTanta University · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present study aimed to detect the prevalence of NOTCH1 c.7541-7542delCT mutation in Egyptian CLL patients using HRM assay and to assess its relation to patients' survival. The study included 50 newly diagnosed treatment-naïve CLL patients and 50 age and sex matched healthy controls. NOTCH1 c.7541-7542delCT mutation was detected using High-resolution melting (HRM) assay and direct Sanger sequencing. Outcome parameters included progression free survival (PFS) and overall survival (OS). NOTCH1 c.7541-7542delCT mutation was detected in 5 (10.0%) of CLL patients. No controls had NOTCH1 c.7541-7542delCT mutation. Similar results were obtained by direct Sanger sequencing yielding a sensitivity and specificity of 100.0% for HRM in detection of NOTCH1 c.7541-7542delCT mutation in the studied patients. In univariate analysis, predictors of OS included Trisomy 12, high LDH, presence of NOTCH1 c.7541-7542delCT mutation and lack of CR. In multivariate analysis, only lack of CR was found as a significant predictor of OS. HRM analysis is a sensitive method for detection of NOTCH1 c.7541-7542delCT mutation in CLL patients. This mutation may be linked to poor disease prognosis.

Indexed as

Chronic lymphocytic leukemiaDNA sequencingHigh-resolution melting analysisNOTCH1 mutationsPrognosis

Identifiers

PMID36258720
PMCPMC9569265
OpenAlexW4223497031

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.