Method development for the determination of seven ginsenosides in three Panax ginseng reference materials via liquid chromatography with tandem mass spectrometry.

Hugh V Hayes, Walter B Wilson, Catherine A Rimmer

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Article in Analytical and bioanalytical chemistry, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in

1citing papers in PubMed

0.2field-weighted citation impact, top 51% of its field

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1 citing paper in PubMed, 2 citations in OpenAlex.

Determination of Toxic Elements in Botanical Dietary Supplement Ingredient Reference Materials.Journal of AOAC International · 2024
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5 · Who and what money

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3 authors at 2 institutions in 1 country.

Hugh V HayesChemical Sciences Division, Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD, 20899, USA. hugh.hayes@nist.gov.

Walter B WilsonChemical Sciences Division, Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD, 20899, USA.

Catherine A RimmerChemical Sciences Division, Material Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, MD, 20899, USA.

Material Measurement Laboratory · USNational Institute of Standards and Technology · US

Funding

Intramural NIST DOC 9999-NIST

6 · The paper itself

Abstract

A new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the analysis of ginsenosides in three Panax ginseng reference materials (RMs). Extraction procedures were optimized to recover neutral and malonyl-ginsenosides using a methanol-water extraction under basic conditions. Optimized mass fragmentation transitions were obtained for the development of a multiple reaction monitoring (MRM) detection method with electrospray ionization in negative and positive ion mode. Mass fraction values were determined for ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf, and Rg1 in the three ginseng materials (rhizomes, extract, and an oral dosage form). Quantitation of these seven compounds was accomplished with 4-methylestradiol and SRM 3389 Ginsenoside Calibration Solution serving as an internal standard (IS) and calibration standards, respectively. Mass fraction values for the seven ginsenosides ranged from 1.27 mg/g to 21.42 mg/g, 3.25 mg/g to 35.81 mg/g, and 0.56 mg/g to 2.51 mg/g for SRM 3384, SRM 3385, and RM 8664, respectively.

Indexed as

GinsenosidesPanaxChromatography, High Pressure LiquidChromatography, LiquidRhizomeTandem Mass SpectrometryGinsenosidesDietary supplementationGinsengLiquid chromatography-tandem mass spectrometryMethod developmentNatural productsReference materials

Identifiers

PMID36258086

DOI10.1007/s00216-022-04378-9

PMCPMC9827472

OpenAlexW4306750800

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.