ArticleScientific reports2022
Hindlimb unloading induces time-dependent disruption of testicular histology in mice.
Article in Scientific reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- Protective effects of Withania somnifera (Ashwagandha) against chronic unpredictable mild stress-induced testicular damage in adolescent rats.Journal of molecular histology · 2026Article
- 4-Phenylbutyrate mitigates renal pathology linked to ER stress related pathways in C57BL/6J mice with hindlimb unloading.Scientific reports · 2026Article
- A systematic review of microgravity on reproductive systems: Implications for space biology and human health.iScience · 2026Review
- Pharmacological inhibition of endoplasmic reticulum stress mitigates osteoporosis in a mouse model of hindlimb suspension.Scientific reports · 2024Article
- Adaptation to full weight-bearing following disuse in rats: The impact of biological sex on musculoskeletal recovery.Physiological reports · 2024Article
- Influence of gonadectomy on muscle health in micro- and partial-gravity environments in rats.Journal of applied physiology (Bethesda, Md. : 1985) · 2023Article
- Effect of Microgravity on the Gut Microbiota Bacterial Composition in a Hindlimb Unloading Model.Life (Basel, Switzerland) · 2022Article
- Complete genome analysis ofFrontiers in veterinary science · 2022Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mechanical unloading of the body in the hindlimb unloaded (HU) mice induces pathology in multiple organs, but the effects on testes are poorly characterized. We investigated the histology and Raman spectroscopy of the mouse testes following HU condition. We divided male, c57BL/6j mice into ground-based controls or experimental groups for two and four weeks of HU. The testes tissues were dissected after euthanasia to investigate histological and Raman spectroscopic analysis. We found an HU-induced atrophy of testes irrespective of the time duration (p < 0.05). Histological analysis revealed that the HU induced epithelial thinning, luminal widening, and spermatozoa decline in the seminiferous tubules of the mouse testes. In addition, we found a thickening of the epididymal epithelia and tunica albuginea. These changes were accompanied by a generalized depression in the Raman spectra, indicating an altered concentration and/or orientation of several molecules. We also report reduced signal intensities of hydroxyproline and tryptophan, potentially contributing to testicular pathology during HU. Taken together, our findings indicate that the two or four weeks of HU induce disruption of testicular architecture and molecular phenotypes. Our results may have implications for understanding and/or treating male infertility associated with prolonged bed rest and spaceflight.
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