ArticleNature communications2022
Structural insight into Tn3 family transposition mechanism.
Article in Nature communications, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed.
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- Soil management practices shape the abundance, diversity, and spread of antimicrobial resistance.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Characteristics of an NDM-1-producing Klebsiella pneumoniae strain belonging to ST105.Antonie van Leeuwenhoek · 2026Article
- Ulva fasciata litter decomposition fuels microbial restructuring and antibiotic resistance proliferation in mariculture sediment.Antonie van Leeuwenhoek · 2026Article
- Genome Evolution in Plant Pathogenic Bacteria.Genome biology and evolution · 2026Review
- Genomic insight into the antibiotic-resistant determinants ofFrontiers in microbiology · 2026Article
- Molecular insights into antimicrobial resistance in human bacterial pathogens: mechanisms, resistance genes, and translational diagnostic applications.Frontiers in microbiology · 2026Review
- Multilayered molecular mechanisms and regulatory networks of aminoglycoside resistance in Enterobacteriaceae.Frontiers in cellular and infection microbiology · 2026Review
- Mobile genetic elements inJournal of bacteriology · 2025Review
- Metatranscriptomic time series insight into antibiotic resistance genes and mobile genetic elements in wastewater systems under antibiotic selective pressure.BMC microbiology · 2025Article
- Metagenomic insights into the resistome, mobilome, and virulome of dogs with diverse lifestyles.Animal microbiome · 2024Article
- Transposition with Tn3-family elements occurs through interaction with the host β-sliding clamp processivity factor.Nucleic acids research · 2024Article
- AFM-based force spectroscopy unravels stepwise formation of the DNA transposition complex in the widespread Tn3 family mobile genetic elements.Nucleic acids research · 2023Article
- Insertion sequence contributes to the evolution and environmental adaptation of Acidithiobacillus.BMC genomics · 2023Article
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6 authors.
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Abstract
Transposons are diverse mobile genetic elements that play the critical role as genome architects in all domains of life. Tn3 is a widespread family and among the first identified bacterial transposons famed for their contribution to the dissemination of antibiotic resistance. Transposition within this family is mediated by a large TnpA transposase, which facilitates both transposition and target immunity. Howtever, a structural framework required for understanding the mechanism of TnpA transposition is lacking. Here, we describe the cryo-EM structures of TnpA from Tn4430 in the apo form and paired with transposon ends before and after DNA cleavage and strand transfer. We show that TnpA has an unusual architecture and exhibits a family specific regulatory mechanism involving metamorphic refolding of the RNase H-like catalytic domain. The TnpA structure, constrained by a double dimerization interface, creates a peculiar topology that suggests a specific role for the target DNA in transpososome assembly and activation.
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