ArticleJournal of the American Chemical Society2022
Orthogonal Enzyme-Driven Timers for DNA Strand Displacement Reactions.
Article in Journal of the American Chemical Society, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Enzymatic DNA Reaction Networks for Orchestrating Stimuli-Dependent Temporal Molecular Pulse.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Enzyme-Free DNA Logic Circuit with Single-Color Readout for Dual Biomarker Detection.Applied biochemistry and biotechnology · 2026Article
- Design and Characterization of DX-Tile DNA Nanostar-Based Hydrogels.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Steric regulation of CRISPR/Cas12a trans-cleavage kinetics via split-activator extensions.Nucleic acids research · 2026Article
- Stimuli-Responsive Chemical Systems That Mimic Biological Dynamics.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Review
- Sustained, Reversible, and Adaptive Non-Equilibrium Steady States of a Dissipative DNA-Based System.Angewandte Chemie (International ed. in English) · 2025Article
- Kinetics and Activation Strategies in Toehold-Mediated and Toehold-Free DNA Strand Displacement.Biosensors · 2025Review
- Mismatch-Assisted Toehold Exchange Cascades for Magnetic Nanoparticle-based Nucleic Acid Diagnostics.JACS Au · 2025Article
- Designing a DNA synchronizer for compact single-rail DNA logic circuits.Science advances · 2025Article
- Mismatch-Induced Toehold-Free Strand Displacement Used to Control a DNA Nanodevice.ACS synthetic biology · 2025Article
- Regulation of CRISPR trans-cleavage activity by an overhanging activator.Nucleic acids research · 2025Article
- High-Speed Sequential DNA Computing Using a Solid-State DNA Origami Register.ACS central science · 2024Article
- Synthetic Protein-to-DNA Input Exchange for Protease Activity Detection Using CRISPR-Cas12a.Analytical chemistry · 2024Article
- Enzyme-Responsive DNA Condensates.Journal of the American Chemical Society · 2024Article
- Temporally controlled multistep division of DNA droplets for dynamic artificial cells.Nature communications · 2024Article
- An Exo III-powered closed-loop DNA circuit architecture for biosensing/imaging.Mikrochimica acta · 2024Article
- Wireless real-time monitoring of oestradiol in sweat.Nature nanotechnology · 2024Article
- DNA Reaction System That Acquires Classical Conditioning.ACS synthetic biology · 2024Article
- A DNA rotary nanodevice operated by enzyme-initiated strand resetting.Chemical communications (Cambridge, England) · 2024Article
- Timed Pulses in DNA Strand Displacement Reactions.Journal of the American Chemical Society · 2023Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we demonstrate a strategy to rationally program a delayed onset of toehold-mediated DNA strand displacement reactions (SDRs). The approach is based on blocker strands that efficiently inhibit the strand displacement by binding to the toehold domain of the target DNA. Specific enzymatic degradation of the blocker strand subsequently enables SDR. The kinetics of the blocker enzymatic degradation thus controls the time at which the SDR starts. By varying the concentration of the blocker strand and the concentration of the enzyme, we show that we can finely tune and modulate the delayed onset of SDR. Additionally, we show that the strategy is versatile and can be orthogonally controlled by different enzymes each specifically targeting a different blocker strand. We designed and established three different delayed SDRs using RNase H and two DNA repair enzymes (formamidopyrimidine DNA glycosylase and uracil-DNA glycosylase) and corresponding blockers. The achieved temporal delay can be programed with high flexibility without undesired leak and can be conveniently predicted using kinetic modeling. Finally, we show three possible applications of the delayed SDRs to temporally control the ligand release from a DNA nanodevice, the inhibition of a target protein by a DNA aptamer, and the output signal generated by a DNA logic circuit.
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