Evidence map›Paper›PMID 36255705›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Hematopoietic Stem and Progenitor Cell Identification and Transplantation in Zebrafish.

Ellen Fraint, Peng Lv, Feng Liu, Teresa V Bowman, Owen J Tamplin

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Ellen FraintDepartment of Pediatrics (Pediatric Hematology/Oncology and Cellular Therapy) and Department of Developmental and Molecular Biology, Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY, USA.
Peng LvState Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Feng LiuState Key Laboratory of Membrane Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Teresa V BowmanGottesman Institute for Stem Cell Biology and Regenerative Medicine, Department of Developmental and Molecular Biology, and Department of Medicine (Oncology), Albert Einstein College of Medicine and Montefiore Medical Center, Bronx, NY, USA.
Owen J TamplinDepartment of Cell and Regenerative Biology, University of Wisconsin-Madison, Madison, WI, USA. tamplin@wisc.edu.

Funding

Improving the interaction of hematopoietic stem cells with the perivascular niche to promote engraftmentR01HL142998 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI TAMPLIN, OWEN JAMES · 2018 to 2022
$2.3M
Crosstalk of Splicing and Signaling in HSPC fate choicesR01DK121738 · NIDDK · ALBERT EINSTEIN COLLEGE OF MEDICINE · PI BOWMAN, TERESA V · 2020 to 2023
$1.6M
Live imaging to determine the behavior of endogenous hematopoietic stem cellsK01DK103908 · NIDDK · UNIVERSITY OF ILLINOIS AT CHICAGO · PI TAMPLIN, OWEN JAMES · 2015 to 2017
$479k
NHLBI NIH HHS R01 HL142998NIDDK NIH HHS K01 DK103908NIDDK NIH HHS R01 DK121738
6 · The paper itself

Abstract

The zebrafish as a model organism is well known for its versatile genetics, rapid development, and straightforward live imaging. It is an excellent model to study hematopoiesis because of its highly conserved ontogeny and gene regulatory networks. Recently developed highly specific transgenic reporter lines have allowed direct imaging and tracking of hematopoietic stem and progenitor cells (HSPCs) in live zebrafish. These reporter lines can also be used for fluorescence-activated cell sorting (FACS) of HSPCs. Similar to mammalian models, HSPCs can be transplanted to reconstitute the entire hematopoietic system of zebrafish recipients. However, the zebrafish provides unique advantages to study HSPC biology, such as transplants into embryos and high-throughput chemical screening. This chapter will outline the methods needed to identify, isolate, and transplant HSPCs in zebrafish.

Indexed as

Hematopoietic Stem Cell TransplantationZebrafishAnimalsAnimals, Genetically ModifiedHematopoiesisHematopoietic Stem CellsMammalsZebrafish ProteinsZebrafish ProteinsHematopoietic stem and progenitor cells (HSPCs)HSPC transplantationLive imagingTransgenic reporter linesZebrafish

Identifiers

PMID36255705
PMCPMC11984326

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.