Evidence map›Paper›PMID 36253877›Full record

ArticleVeterinary medicine and science2022

Evaluation of in vitro antibacterial effect of essential oil and some herbal plant extract used against mastitis pathogens.

Safia Arbab, Hanif Ullah, Iqra Bano, Ka Li, Inam Ul Hassan, Weiwei Wang, Abdul Qadeer, Jiyu Zhang

Open access · goldAbstract read
In one paragraph

Article in Veterinary medicine and science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
4.3field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 30 citations in OpenAlex.

  1. Article
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  5. Antimicrobial activity of brown propolis ethanolic extract and its application in intramammary formulation for the treatment of bovine mastitis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026
    Article
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  8. Review
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  15. Antibacterial Effects of Essential Oils onPharmaceuticals (Basel, Switzerland) · 2024
    Article
  16. Article
  17. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 2 countries.

Safia ArbabKey Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou, China.ORCID 0000-0002-9940-9708
Hanif UllahWest China School of Nursing/ West China Hospital, Sichuan University, Chengdu, China.
Iqra BanoFaculty of Bioscience, Department of Physiology and Biochemistry, Shaheed Benazir Bhutto University of Veterinary & Animal Sciences, Sakrand, Pakistan.
Ka LiWest China School of Nursing/ West China Hospital, Sichuan University, Chengdu, China.
Inam Ul HassanDepartment of Microbiology, Hazara University Manshera, Khyber Pakhtunkhwa, Pakistan.
Weiwei WangKey Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou, China.
Abdul QadeerKey Laboratory of Animal Parasitology of Ministry of Agriculture and Rural Affairs, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, China.
Jiyu ZhangKey Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture, Lanzhou, China.
Lanzhou Institute of Husbandry and Pharmaceutical Sciences · CNSichuan University · CNHazara University · PKShaheed Benazir Bhutto University · PKShanghai Veterinary Research Institute · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMastitis in dairy cattle is a highly prevalent infectious disease, caused by various pathogens, mainly Staphylococcu aureus and Escherichia Coli, considerable economic loss worldwide.

objectivesThe aim of this study was to evaluate the in vitro activity of Herbal plants used against S. aureus and E. coli bacteria which are the causative agents of mastitis.

methodsTherefore, in this study we investigate the antimicrobial effect of plant to evaluate the in vitro antibacterial activity of squaw mint (Mentha pulegium L., Lamiaceae family), catnip (Nepeta cataria L., Lamiaceae), lemon balm (Melissa officinalis L., Lamiaceae), for mastitis treatment. Solutions prepared in fixed oils, against S. aureus and E. coli bacteria which are the main agents of mastitis. Isolation and antibiotic susceptibility analyses of milk samples taken from 100 subclinical mastitis dairy cows were performed. The antibacterial properties of the solutions were analysed by a disk diffusion method.

resultsIn the bacterial isolation, S. aureus was determined 97.7% and E. coli 53.5% positive of cows with mastitis. Antibacterial susceptibility test of the Lemon balm extract and essential oil showed maximum zone of inhibition against S. aureus 30 µl (23 mm), followed by 20 µl (19 mm), E. coli (19 mm) and 10 µl (5-7 mm), of the same extract against the Gram-positive bacteria. The ethanol extracts show the similar activity against the Gram-negative bacteria at 30, 20, and 10 µl (18-20 mm). Followed by S. aureus, when the zone areas for the susceptible solutions (Lemon balm, and essential oil) and the control group were compared, determined that there was little difference between for S. aureus and E. coli.

conclusionsThis study hence indicated that in vitro cultured plantlets of lemon balm and peppermint oil can be used as the alternative method for production of mastitis and cheap source its precursor with antimicrobial activities.

Indexed as

Cattle DiseasesMastitis, BovineOils, VolatileAnimalsAnti-Bacterial AgentsCattleEscherichia coliFemalePlant ExtractsStaphylococcus aureusAnti-Bacterial AgentsOils, VolatilePlant Extractsessential oilherbal plantsin vitrosubclinical mastitis

Identifiers

PMID36253877
PMCPMC9677380
OpenAlexW4306658502

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.