Evidence map›Paper›PMID 36253859›Full record

ArticleVirology journal2022

Identification of a novel interaction site between the large hepatitis delta antigen and clathrin that regulates the assembly of genotype III hepatitis delta virus.

Wei-Chung Chiou, Hsu-Feng Lu, Jui-Chieh Chen, Yu-Heng Lai, Ming-Fu Chang, Yuan-Li Huang, Ni Tien, Cheng Huang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology journal, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
  3. Structure and nucleic acid interactions of the SProceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  4. Virus infection and vesicle trafficking.Frontiers in immunology · 2025
    Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 7 institutions in 1 country.

Wei-Chung Chiou *Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming Chiao Tung University, No. 155, Sec. 2, Linong St., Beitou District, Taipei, 11221, Taiwan.
Hsu-Feng Lu *Department of Medical Laboratory Science and Biotechnology, Asia University, Taichung, 41354, Taiwan.
Jui-Chieh ChenDepartment of Biochemical Science and Technology, National Chiayi University, Chiayi, 60004, Taiwan.
Yu-Heng LaiDepartment of Chemistry, Chinese Culture University, Taipei, 11114, Taiwan.
Ming-Fu ChangInstitute of Biochemistry and Molecular Biology, School of Medicine, National Taiwan University, Taipei, 10051, Taiwan.
Yuan-Li HuangDepartment of Medical Laboratory Science and Biotechnology, Asia University, Taichung, 41354, Taiwan.
Ni TienDepartment of Laboratory Medicine, China Medical University Hospital, Taichung, 40402, Taiwan.
Cheng HuangDepartment of Biotechnology and Laboratory Science in Medicine, National Yang Ming Chiao Tung University, No. 155, Sec. 2, Linong St., Beitou District, Taipei, 11221, Taiwan. chengh@ym.edu.tw.
National Yang Ming Chiao Tung University · TWAsia University · TWChina Medical University · TWChina Medical University Hospital · TWChinese Culture University · TWNational Chiayi University · TWNational Taiwan University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHepatitis delta virus (HDV), a satellite virus of hepatitis B virus (HBV), is a small, defective RNA virus strongly associated with the most severe form of hepatitis and progressive chronic liver disease and cirrhosis. Chronic hepatitis D, resulting from HBV/HDV coinfection, is considered to be the most severe form of viral hepatitis and affects 12-20 million people worldwide. Involved in the endocytosis and exocytosis of cellular and viral proteins, clathrin contributes to the pathogenesis and morphogenesis of HDV. Previously, we demonstrated that HDV-I and -II large hepatitis delta antigens (HDAg-L) possess a putative clathrin box that interacts with clathrin heavy chain (CHC) and supports HDV assembly.

methodsVirus assembly and vesicular trafficking of HDV virus-like particles (VLPs) were evaluated in Huh7 cells expressing HDV-I, -II and -III HDAg-L and hepatitis B surface antigen (HBsAg). To elucidate the interaction motif between HDAg-L and CHC, site-directed mutagenesis was performed to introduce mutations into HDAg-L and CHC and analyzed using coimmunoprecipitation or pull-down assays.

resultsComparable to HDV-I virus-like particles (VLPs), HDV-III VLPs were produced at a similar level and secreted into the medium via clathrin-mediated post-Golgi vesicular trafficking. Mutation at F27 or E33 of CHC abolished the binding of CHC to the C-terminus of HDV-III HDAg-L. Mutation at W207 of HDV-III HDAg-L inhibited its association with CHC and interfered with HDV-III VLP formation. We elucidated mechanism of the binding of HDV-III HDAg-L to CHC and confirmed the pivotal role of clathrin binding in the assembly of genotype III HDV.

conclusionsA novel W box which was identified at the C terminus of HDV-III HDAg-L is known to differ from the conventional clathrin box but also interacts with CHC. The novel W box of HDAg-L constitutes a new molecular target for anti-HDV-III therapeutics.

Indexed as

Hepatitis B Surface AntigensHepatitis Delta VirusClathrinClathrin Heavy ChainsGenotypeHepatitis B virusHepatitis delta AntigensHumansRNA, ViralViral ProteinsVirus ReplicationClathrinClathrin Heavy ChainsHepatitis B Surface AntigensHepatitis delta Antigenshepatitis delta virus large antigenRNA, ViralViral ProteinsClathrin boxClathrin heavy chainHDV genotypesHepatitis delta antigenW box

Identifiers

PMID36253859
PMCPMC9578201
OpenAlexW4306408042

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.