ReviewNature protocols2023
Direct delivery and fast-treated Agrobacterium co-culture (Fast-TrACC) plant transformation methods for Nicotiana benthamiana.
Review in Nature protocols, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 63 citations in OpenAlex.
- An Efficient Regeneration and Transient Transformation System for the Desert HalophytePlants (Basel, Switzerland) · 2026Article
- Bibliometric-Based Analysis of Global Trends and Collaborative Networks in Plant Genetic Engineering (1994-2024).Plant biotechnology journal · 2026Review
- Advances in the use of morphogenic regulators and peptide regenerating factors for boosting plant transformation and genome editing.Frontiers in plant science · 2026Review
- Time-resolved transcriptomic analysis suggests candidate hub gene modules and putative regulatory pathways in tobacco under dicamba stress.Frontiers in plant science · 2026Article
- The Agrobacterium-mediated genetic transformation: a gateway for efficient CRISPR/Cas9 gene editing in leguminous.Transgenic research · 2025Review
- Studies on the mother flower carnation: past, present, and future.Horticulture research · 2025Article
- Genome-Wide Identification, Evolution and Expression Analysis ofPlants (Basel, Switzerland) · 2025Article
- Non-tissue culture genetic modifications for plant improvement.Plant molecular biology · 2025Review
- In planta genome editing in citrus facilitated by co-expression of CRISPR/Cas and developmental regulators.The Plant journal : for cell and molecular biology · 2025Article
- Current Advancement and Future Prospects in Simplified Transformation-Based Plant Genome Editing.Plants (Basel, Switzerland) · 2025Review
- Success and limitations in adaptation of Fast-TrACC tissue culture-independent transformation in coffee, cotton, and tree tobacco.PloS one · 2025Article
- Unlocking regeneration potential: harnessing morphogenic regulators and small peptides for enhanced plant engineering.The Plant journal : for cell and molecular biology · 2025Review
- Soybean genomics research community strategic plan: A vision for 2024-2028.The plant genome · 2024Article
- Improved Protocol for EfficientPlants (Basel, Switzerland) · 2024Article
- Advancements in delivery strategies and non-tissue culture regeneration systems for plant genetic transformation.Advanced biotechnology · 2024Review
- Appreciating animal induced pluripotent stem cells to shape plant cell reprogramming strategies.Journal of experimental botany · 2024Review
- Plant regeneration in the new era: from molecular mechanisms to biotechnology applications.Science China. Life sciences · 2024Review
- Robust soybean leaf agroinfiltration.Plant cell reports · 2024Article
- Article
- A comprehensive review of in planta stable transformation strategies.Plant methods · 2024Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
There is an expanding need to modify plant genomes to create new plant germplasm that advances both basic and applied plant research. Most current methods for plant genome modification involve regenerating plants from genetically modified cells in tissue culture, which is technically challenging, expensive and time consuming, and works with limited plant species or genotypes. Herein, we describe two Agrobacterium-based methods for creating genetic modifications on either sterilely grown or soil-grown Nicotiana benthamiana plants. These methods use developmental regulators (DRs), gene products that influence cell division and differentiation, to induce de novo meristems. Genome editing reagents, such as the RNA-guided endonuclease Cas9, may be co-delivered with the DRs to create shoots that transmit edits to the next generation. One method, called fast-treated Agrobacterium co-culture (Fast-TrACC), delivers DRs to seedlings grown aseptically; meristems that produce shoots and ultimately whole plants are induced. The other approach, called direct delivery (DD), involves delivering DRs to soil-grown plants from which existing meristems have been removed; the DRs promote the formation of new shoots at the wound site. With either approach, if transgene cassettes and/or gene editing reagents are provided, these induced, de novo meristems may be transgenic, edited or both. These two methods offer alternative approaches for generating novel plant germplasm that are cheaper and less technically challenging and take less time than standard approaches. The whole procedure from transfer DNA (T-DNA) assembly to recovery of edited plants can be completed in ~70 d for both DD and Fast-TrACC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.