Evidence map›Paper›PMID 36252818›Full record

ArticleNeurochemistry international2022

MMP-12 knockdown prevents secondary brain damage after ischemic stroke in mice.

Vijay Arruri, Anil K Chokkalla, Soomin Jeong, Bharath Chelluboina, Suresh L Mehta, Krishna Kumar Veeravalli, Raghu Vemuganti

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neurochemistry international, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Review
  5. Article
  6. Loss of Epitranscriptomic Modification NTranslational stroke research · 2025
    Article
  7. Article
  8. Review
  9. Article
  10. Review
  11. Article
  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Vijay ArruriDepartment of Neurological Surgery, University of Wisconsin-Madison, Madison, WI, USA.
Anil K ChokkallaDepartment of Neurological Surgery, University of Wisconsin-Madison, Madison, WI, USA.
Soomin JeongDepartment of Neurological Surgery, University of Wisconsin-Madison, Madison, WI, USA; Neuroscience Training Program, University of Wisconsin-Madison, Madison, WI, USA.
Bharath ChelluboinaDepartment of Neurological Surgery, University of Wisconsin-Madison, Madison, WI, USA.
Suresh L MehtaDepartment of Neurological Surgery, University of Wisconsin-Madison, Madison, WI, USA.
Krishna Kumar VeeravalliDepartment of Cancer Biology and Pharmacology, University of Illinois College of Medicine at Peoria, Peoria, IL, USA; Department of Neurosurgery, University of Illinois College of Medicine at Peoria, Peoria, IL, USA; Department of Pediatrics, University of Illinois College of Medicine at Peoria, Peoria, IL, USA; Department of Neurology, University of Illinois College of Medicine at Peoria, Peoria, IL, USA. Electronic address: krishnav@uic.edu.
Raghu VemugantiDepartment of Neurological Surgery, University of Wisconsin-Madison, Madison, WI, USA; Neuroscience Training Program, University of Wisconsin-Madison, Madison, WI, USA; William S. Middleton Veterans Administration Hospital, Madison, WI, USA. Electronic address: vemuganti@neurosurgery.wisc.edu.
University of Wisconsin–Madison · USUniversity of Illinois Urbana-Champaign · USUniversity of Wisconsin System · US

Funding

MMP-12 and post-stroke brain damageR01NS102573 · NINDS · UNIVERSITY OF ILLINOIS AT CHICAGO · PI VEERAVALLI, KRISHNA KUMAR · 2018 to 2022
$1.8M
BLRD Research Career Scientist Award ApplicationIK6BX005690 · VA · WM S. MIDDLETON MEMORIAL VETERANS HOSP · PI VEMUGANTI, RAGHU · 2021 to 2025
–
BLRD VA IK6 BX005690NINDS NIH HHS R01 NS102573
6 · The paper itself

Abstract

We previously reported that increased expression of matrix metalloproteinase-12 (MMP-12) mediates blood-brain barrier disruption via tight junction protein degradation after focal cerebral ischemia in rats. Currently, we evaluated whether MMP-12 knockdown protects the post-stroke mouse brain and promotes better functional recovery. Adult male mice were injected with negative siRNA or MMP-12 siRNA (intravenous) at 5 min of reperfusion following 1 h transient middle cerebral artery occlusion. MMP-12 knockdown significantly reduced the post-ischemic infarct volume and improved motor and cognitive functional recovery. Mechanistically, MMP-12 knockdown ameliorated degradation of tight junction proteins zonula occludens-1, claudin-5, and occludin after focal ischemia. MMP-12 knockdown also decreased the expression of inflammatory mediators, including monocyte chemoattractant protein-1, tumor necrosis factor-α, and interleukin-6, and the expression of apoptosis marker cleaved caspase-3 after ischemia. Overall, the present study indicates that MMP-12 promotes secondary brain damage after stroke and hence is a promising stroke therapeutic target.

Indexed as

Brain InjuriesBrain IschemiaIschemic StrokeStrokeAnimalsBlood-Brain BarrierInfarction, Middle Cerebral ArteryMaleMatrix Metalloproteinase 12MiceOccludinRNA, Small InterferingMatrix Metalloproteinase 12OccludinRNA, Small InterferingInflammationIschemia-reperfusionMatrix metalloproteinasesNeuroprotectionTight junction proteins

Identifiers

PMID36252818
PMCPMC9907318
OpenAlexW4306169158

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.