ArticleMicrobiology spectrum2022
Nanopore Sequencing Using the Full-Length 16S rRNA Gene for Detection of Blood-Borne Bacteria in Dogs Reveals a Novel Species of Hemotropic Mycoplasma.
Article in Microbiology spectrum, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 37 citations in OpenAlex.
- Transforming gastrointestinal helminth parasite identification in vertebrate hosts with metabarcoding: a systematic review.Parasites & vectors · 2024Pooled it
- Gallic Acid Attenuates Ifosfamide-Induced Gut Microbiota Dysbiosis: A Full-Length 16S rRNA Amplicon Sequencing Study.Microorganisms · 2026Article
- Hemotropic mycoplasmas and Bartonella spp. in dogs from Southern Italy: prevalence and risk factors.Veterinary research communications · 2026Article
- Development and evaluation of a multiplex PCR-based dual-platform targeted sequencing framework for precise differentiation of lumpy skin disease virus.Frontiers in microbiology · 2026Article
- Hidden hemoplasma species within the "Candidatus Mycoplasma haemominutum" lineage in Thai cats revealed by analyses of two independent genetic markers.Parasites & vectors · 2025Article
- Genetic characterization of zoonoticMicrobiology spectrum · 2025Article
- Temperature-dependent shifts in gut microbiota and metabolome of olive flounder (Paralichthys olivaceus): implications for cold-water aquaculture expansion and probiotic applications.Animal microbiome · 2025Article
- Evaluating the efficiency of 16S-ITS-23S operon sequencing for species level resolution in microbial communities.Scientific reports · 2025Article
- Expanding the use of circulating microbiome in fish: contrast between the gut and blood microbiome ofISME communications · 2025Article
- Nanopore sequencing in veterinary medicine: from concepts to clinical applications.Frontiers in cellular and infection microbiology · 2025Review
- Metabarcoding using nanopore sequencing enables identification of diverse and zoonotic vector-borne pathogens from neglected regions: A case study investigating dogs from Bhutan.One health (Amsterdam, Netherlands) · 2024Article
- The influence of air pollution on skin microbiome: a link to skin barrier dysfunction.Archives of dermatological research · 2024Article
- GROND: a quality-checked and publicly available database of full-length 16S-ITS-23S rRNA operon sequences.Microbial genomics · 2024Article
- Development and validation of a long-read metabarcoding platform for the detection of filarial worm pathogens of animals and humans.BMC microbiology · 2024Article
- Next-generation sequencing metabarcoding assays reveal diverse bacterial vector-borne pathogens of Mongolian dogs.Current research in parasitology & vector-borne diseases · 2024Article
- Dogs are reservoir hosts of the zoonoticOne health (Amsterdam, Netherlands) · 2023Article
- Rapid metagenomic sequencing for diagnosis and antimicrobial sensitivity prediction of canine bacterial infections.Microbial genomics · 2023Article
- Metagenomics of culture isolates and insect tissue illuminate the evolution ofMicrobial genomics · 2023Article
- Transmission of haemotropic mycoplasma in the absence of arthropod vectors within a closed population of dogs on ectoparasiticides.Scientific reports · 2023Article
- Gut microbiome profiling of neonates using Nanopore MinION and Illumina MiSeq sequencing.Frontiers in microbiology · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dogs across the globe are afflicted by diverse blood- and vector-borne bacteria (VBB), many of which cause severe disease and can be fatal. Diagnosis of VBB infections can be challenging due to the low concentration of bacteria in the blood, the frequent occurrence of coinfections, and the wide range of known, emerging, and potentially novel VBB species encounterable. Therefore, there is a need for diagnostics that address these challenges by being both sensitive and capable of detecting all VBB simultaneously. We detail the first employment of a nanopore-based sequencing methodology conducted on the Oxford Nanopore Technologies (ONT) MinION device to accurately elucidate the "hemobacteriome" from canine blood through sequencing of the full-length 16S rRNA gene. We detected a diverse range of important canine VBB, including Ehrlichia canis, Anaplasma platys, Mycoplasma haemocanis, Bartonella clarridgeiae, "
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.