Evidence map›Paper›PMID 36246330›Full record

ArticleFrontiers in veterinary science2022

Inguinal lymph node sample collected by minimally invasive sampler helps to accurately diagnose ASF in dead pigs without necropsy.

Xiaowen Li, Yang Li, Mingyu Fan, Shiran Fan, Wenchao Gao, Jing Ren, Qingyuan Liu, Jingtao Li, Weisheng Wu, Junxian Li and 3 more

Open access · goldAbstract read
In one paragraph

Article in Frontiers in veterinary science, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.6field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Xiaowen LiShandong New Hope Liuhe Agriculture and Animal Husbandry Technology Co., Ltd., (NHLH Academy Swine Research), Dezhou, China.
Yang LiShandong New Hope Liuhe Agriculture and Animal Husbandry Technology Co., Ltd., (NHLH Academy Swine Research), Dezhou, China.
Mingyu FanXiajin New Hope Liuhe Agriculture and Animal Husbandry Co., Ltd., Dezhou, China.
Shiran FanNew Hope Liuhe Co., Ltd., Chengdu, China.
Wenchao GaoShandong New Hope Liuhe Agriculture and Animal Husbandry Technology Co., Ltd., (NHLH Academy Swine Research), Dezhou, China.
Jing RenSwine Health Data and Intelligent Monitoring Project Laboratory, Dezhou University, Dezhou, China.
Qingyuan LiuShandong New Hope Liuhe Agriculture and Animal Husbandry Technology Co., Ltd., (NHLH Academy Swine Research), Dezhou, China.
Jingtao LiNew Hope Liuhe Co., Ltd., Chengdu, China.
Weisheng WuNew Hope Liuhe Co., Ltd., Chengdu, China.
Junxian LiNew Hope Liuhe Co., Ltd., Chengdu, China.
Qiannan YuXiajin New Hope Liuhe Agriculture and Animal Husbandry Co., Ltd., Dezhou, China.
Xinglong WangCollege of Veterinary Medicine, Northwest A&F University, Yangling, China.
Zhichun YanNew Hope Liuhe Co., Ltd., Chengdu, China.
New Hope Liuhe (China) · CNDezhou University · CNNorthwest A&F University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

African swine fever (ASF) is a highly contagious hemorrhagic and transboundary animal disease, and it threatens global food security. A full necropsy to harvest the sample matrices for diagnosis in the farm may lead to contamination of the premises and directly threaten to the herds. In the present study, we compared the ASFV loads of the common samples that can be collected without necropsy. The unmatched nasal, throat, rectal samples were randomly taken using cotton swabs, and inguinal lymph node samples were collected by the minimally invasive samplers from the dead pigs of an ASF field outbreak farm. The ASFV loads of the samples were detected by qPCR and the results suggested that the overall ASFV nucleic acids levels of inguinal lymph node samples were higher than the swabs. What's more, sets of matched nasal swabs, rectal swabs, throat swabs, inguinal lymph nodes, serums, spleens and lungs samples were collected from 15 dead ASFV naturally infected pigs. Similarly, the results showed that inguinal lymph node samples, together with serum, spleen and lungs samples, contained more ASFV nucleic acids than the swabs. Our findings demonstrated that the inguinal lymph node collected by minimally invasive sampler is an ideal tissue for diagnosing ASFV infection in dead pigs without necropsy.

Indexed as

African swine feverdiagnosisinguinal lymph nodeminimally invasive samplerqPCR

Identifiers

PMID36246330
PMCPMC9554536
OpenAlexW4297546047

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.