Evidence map›Paper›PMID 36246012›Full record

ArticleOphthalmology science2021

Increased Corneal Endothelial Cell Migration in Fuchs Endothelial Corneal Dystrophy: A Live Cell Imaging Study.

Stephan Ong Tone, Adam Wylegala, Myriam Böhm, Geetha Melangath, Neha Deshpande, Ula V Jurkunas

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Ophthalmology science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Corneal injury repair and the potential involvement of ZEB1.Eye and vision (London, England) · 2024
    Review
  10. Article
  11. Why Ophthalmology Science?Ophthalmology science · 2021
    Article
  12. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 2 countries.

Stephan Ong ToneCornea Center of Excellence, Schepens Eye Research Institute, Harvard Medical School, Boston, Massachusetts.
Adam WylegalaCornea Center of Excellence, Schepens Eye Research Institute, Harvard Medical School, Boston, Massachusetts.
Myriam BöhmCornea Center of Excellence, Schepens Eye Research Institute, Harvard Medical School, Boston, Massachusetts.
Geetha MelangathCornea Center of Excellence, Schepens Eye Research Institute, Harvard Medical School, Boston, Massachusetts.
Neha DeshpandeCornea Center of Excellence, Schepens Eye Research Institute, Harvard Medical School, Boston, Massachusetts.
Ula V JurkunasCornea Center of Excellence, Schepens Eye Research Institute, Harvard Medical School, Boston, Massachusetts.
Massachusetts Eye and Ear Infirmary · US

Funding

The Role of Oxidative Stress in the Pathogenesis of Fuchs Endothelial Corneal DysR01EY020581 · NEI · SCHEPENS EYE RESEARCH INSTITUTE · PI Ula V. Jurkunas · 2010 to 2026
$8.0M
NEI NIH HHS R01 EY020581
6 · The paper itself

Abstract

Purpose: To investigate if corneal endothelial cells (CECs) in Fuchs endothelial corneal dystrophy (FECD) have altered cellular migration compared with normal controls. Design: Comparative analysis. Materials: Descemet's membrane and CECs derived from patients with FECD undergoing endothelial keratoplasty or normal cadaveric donors. Methods: Ex vivo specimens were used for live cell imaging and generation of immortalized cell lines. Live imaging was performed on FECD and normal CECs and on ex vivo specimens transfected with green fluorescent protein. Migration speeds were determined as a function of cellular density using automated cell tracking. Ex vivo specimens were classified as either FECD or normal low cell density (nonconfluent) or high cell density (confluent). Scratch assay was performed on CECs seeded at high confluence to determine migration speed. Genetic analysis from blood samples or CECs was performed to detect a CTG repeat expansion in the Main Outcome Measures: Mean cell migration speed. Results: Fuchs endothelial corneal dystrophy CECs in low cell density areas displayed increased mean speed (0.391 ± 0.005 μm/minute vs. 0.364 ± 0.005 μm/minute; Conclusions: Fuchs endothelial corneal dystrophy CECs demonstrated increased migration speed compared with normal CECs. Further investigation into the mechanisms of heightened cell migration in FECD is needed and may provide insight into its pathogenesis, as well as having implications on descemetorhexis without endothelial keratoplasty.

Indexed as

CEC, corneal endothelial cellCE, corneal endotheliumCell migrationCorneal endotheliumDescemetorhexis without endothelial keratoplastyDescemet’s stripping onlyDM, Descemet’s membraneDMEK, Descemet's membrane endothelial keratoplastyDWEK, descemetorhexis without endothelial keratoplastyECD, endothelial cell densityECM, extracellular matrixEMT, endothelial-to-mesenchymal transitionFECD, Fuchs endothelial corneal dystrophyFuchs endothelial corneal dystrophyGFP, green fluorescent proteinLNP, lipid nanoparticlePBS, phosphate-buffered salineTCF4, transcription factor 4

Identifiers

PMID36246012
PMCPMC9559113
OpenAlexW3135779565

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.