Evidence map›Paper›PMID 36245800›Full record

ArticleBio-protocol2022

Carbamoyltransferase Enzyme Assay:

Weiwei Yang, Nan Dai, Yu-Cheng Lin, William Johnson, Romualdas Vaisvila, Peter Weigele, Yan-Jiun Lee, Ira Schildkraut, Ivan R Corrêa, Laurence Ettwiller

Open access · greenAbstract read
In one paragraph

Article in Bio-protocol, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 57% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Weiwei YangNew England Biolabs, Ipswich, United States.
Nan DaiNew England Biolabs, Ipswich, United States.
Yu-Cheng LinNew England Biolabs, Ipswich, United States.
William JohnsonNew England Biolabs, Ipswich, United States.
Romualdas VaisvilaNew England Biolabs, Ipswich, United States.
Peter WeigeleNew England Biolabs, Ipswich, United States.
Yan-Jiun LeeNew England Biolabs, Ipswich, United States.
Ira SchildkrautNew England Biolabs, Ipswich, United States.
Ivan R CorrêaNew England Biolabs, Ipswich, United States.
Laurence EttwillerNew England Biolabs, Ipswich, United States.
New England Biolabs (United States) · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nucleic acids in living organisms are more complex than the simple combinations of the four canonical nucleotides. Recent advances in biomedical research have led to the discovery of numerous naturally occurring nucleotide modifications and enzymes responsible for the synthesis of such modifications. In turn, these enzymes can be leveraged towards toolkits for DNA and RNA manipulation for epigenetic sequencing or other biotechnological applications. Here, we present the protocol to obtain purified 5-hydroxymethylcytosine carbamoyltransferase enzymes and the associated assays to convert 5-hydroxymethylcytosine to 5-carbamoyloxymethylcytosine

Indexed as

5-carbamoyloxymethylcytosine5-hydroxymethylcytosineCarbamoyltransferaseDNA modificationRNA modification

Identifiers

PMID36245800
PMCPMC9510146
OpenAlexW4294605455

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.