ArticleVeterinary research2022
Species identification by MALDI-TOF MS and gap PCR-RFLP of non-aureus Staphylococcus, Mammaliicoccus, and Streptococcus spp. associated with sheep and goat mastitis.
Article in Veterinary research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.
- Non-aureus staphylococci and mammaliicocci as a cause of mastitis in domestic ruminants: current knowledge, advances, biomedical applications, and future perspectives - a systematic review.Veterinary research communications · 2023Pooled it
- Phenotypic resistance to life-saving antimicrobials in Gram-positive bacteria isolated from wild birds in Northern Italy: a One Health surveillance perspective.One health (Amsterdam, Netherlands) · 2026Article
- Survival and Behavior of Potential Emerging Foodborne PathogenFood science & nutrition · 2026Article
- Occurrence of third-generation cephalosporin-resistant Escherichia coli in European hedgehogs (Erinaceus europaeus) from a wildlife rescue centre in Lombardy, Northern Italy.Veterinary research communications · 2026Article
- Genomic Insights into Mammaliicoccus sciuri from Subclinical Bovine Mastitis to Unveil Key Resistance, Virulence, Biofilm and Adaptation Traits.Current microbiology · 2026Article
- Comprehensive evaluation of milk biomarkers as indicators of intramammary infection in dairy goats across lactation.Scientific reports · 2026Article
- Nonantibiotic Alternatives for Mastitis in Small Ruminants: A Scoping Review of Evidence, Gaps, and Translational Challenges.Veterinary medicine international · 2026Review
- Development and application of a PCR-RFLP assay revealing widespread distribution of the pyrethroid resistance-associated VGSC V1016G mutation in Aedes albopictus from Guangyuan City, Sichuan Province of China.Parasites & vectors · 2025Article
- Looking for ESKAPE Bacteria: Occurrence and Phenotypic Antimicrobial Resistance Profiles in Wild Birds from Northern and Central Italy Sites.Antibiotics (Basel, Switzerland) · 2025Article
- Relationship between the composition of vaginal bacterial populations and the reproductive stage in captive collared peccaries.Reproduction & fertility · 2025Article
- Resisting the Final Line: Phenotypic Detection of Resistance to Last-Resort Antimicrobials in Gram-Negative Bacteria Isolated from Wild Birds in Northern Italy.Animals : an open access journal from MDPI · 2025Article
- Enterococcus species identified by MALDI-TOF MS in milk from dairy cow mastitis cases and herd surveys.Veterinary research communications · 2025Article
- Prevalence, Antimicrobial Resistance, and Virulence Potential ofAntibiotics (Basel, Switzerland) · 2025Article
- Endogenous Symmetric Dimethylarginine (SDMA) and Asymmetrical Dimethylarginine (ADMA) Levels in Healthy Cows and Cows with Subclinical and Clinical Mastitis-A Comparative Study.Animals : an open access journal from MDPI · 2025Article
- Fecal carriage of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in South American camelids and biosecurity practices among farms in northern Italy.Veterinary research communications · 2025Article
- Use of approved cefazolin based intramammary dry goat treatment and its effects on the milk microbiota.Scientific reports · 2025Article
- Correlating milk cytokines and somatic cell counts to intramammary infections in dairy sheep.Frontiers in veterinary science · 2025Article
- Emerging of Uncommon Chronic Mastitis FromTransboundary and emerging diseases · 2025Article
- The pathogenicity and virulence of the opportunistic pathogenVirulence · 2024Review
- EAVLD 2024 - 7Italian journal of food safety · 2024Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Staphylococci and streptococci are common causes of intramammary infection in small ruminants, and reliable species identification is crucial for understanding epidemiology and impact on animal health and welfare. We applied MALDI-TOF MS and gap PCR-RFLP to 204 non-aureus staphylococci (NAS) and mammaliicocci (NASM) and to 57 streptococci isolated from the milk of sheep and goats with mastitis. The top identified NAS was Staphylococcus epidermidis (28.9%) followed by Staph. chromogenes (27.9%), haemolyticus (15.7%), caprae, and simulans (6.4% each), according to both methods (agreement rate, AR, 100%). By MALDI-TOF MS, 13.2% were Staph. microti (2.9%), xylosus (2.0%), equorum, petrasii and warneri (1.5% each), Staph. sciuri (now Mammaliicoccus sciuri, 1.0%), arlettae, capitis, cohnii, lentus (now M. lentus), pseudintermedius, succinus (0.5% each), and 3 isolates (1.5%) were not identified. PCR-RFLP showed 100% AR for Staph. equorum, warneri, arlettae, capitis, and pseudintermedius, 50% for Staph. xylosus, and 0% for the remaining NASM. The top identified streptococcus was Streptococcus uberis (89.5%), followed by Strep. dysgalactiae and parauberis (3.5% each) and by Strep. gallolyticus (1.8%) according to both methods (AR 100%). Only one isolate was identified as a different species by MALDI-TOF MS and PCR-RFLP. In conclusion, MALDI-TOF MS and PCR-RFLP showed a high level of agreement in the identification of the most prevalent NAS and streptococci causing small ruminant mastitis. Therefore, gap PCR-RFLP can represent a good identification alternative when MALDI-TOF MS is not available. Nevertheless, some issues remain for Staph. haemolyticus, minor NAS species including Staph. microti, and species of the novel genus Mammaliicoccus.
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