Evidence map›Paper›PMID 36241945›Full record

ArticleAntonie van Leeuwenhoek2022

Identification of fungal dihydrouracil-oxidase genes by expression in Saccharomyces cerevisiae.

Jonna Bouwknegt, Aurin M Vos, Raúl A Ortiz Merino, Daphne C van Cuylenburg, Marijke A H Luttik, Jack T Pronk

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Antonie van Leeuwenhoek, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 56% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Jonna Bouwknegt *Department of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629, HZ, Delft, The Netherlands.ORCID http://orcid.org/0000-0002-1110-6463
Aurin M Vos *Department of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629, HZ, Delft, The Netherlands.ORCID http://orcid.org/0000-0002-8836-3951
Raúl A Ortiz MerinoDepartment of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629, HZ, Delft, The Netherlands.ORCID http://orcid.org/0000-0003-4186-8941
Daphne C van CuylenburgDepartment of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629, HZ, Delft, The Netherlands.
Marijke A H LuttikDepartment of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629, HZ, Delft, The Netherlands.ORCID http://orcid.org/0000-0002-9798-8072
Jack T PronkDepartment of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629, HZ, Delft, The Netherlands. j.t.pronk@tudelft.nl.ORCID http://orcid.org/0000-0002-5617-4611
Delft University of Technology · NL

Funding

H2020 European Research Council 694633
6 · The paper itself

Abstract

Analysis of predicted fungal proteomes revealed a large family of sequences that showed similarity to the Saccharomyces cerevisiae Class-I dihydroorotate dehydrogenase Ura1, which supports synthesis of pyrimidines under aerobic and anaerobic conditions. However, expression of codon-optimised representatives of this gene family, from the ascomycete Alternaria alternata and the basidiomycete Schizophyllum commune, only supported growth of an S. cerevisiae ura1Δ mutant when synthetic media were supplemented with dihydrouracil. A hypothesis that these genes encode NAD(P)

Indexed as

Hydrogen PeroxideSaccharomyces cerevisiaeCell ExtractsGenes, FungalHydrogenNADProteomeThymineUracilCell ExtractsdihydrouracilHydrogenHydrogen PeroxideNADProteomeThymineUracil5-fluorodihydrouracilCounter-selectable marker genesDihydroorotate dehydrogenaseDihydropyrimidine dehydrogenaseDihydropyrimidine oxidaseDihydrothymine

Identifiers

PMID36241945
PMCPMC9585004
OpenAlexW4306252690

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.