Evidence map›Paper›PMID 36240778›Full record

ArticleCancer cell2022

Spatial epitope barcoding reveals clonal tumor patch behaviors.

Xavier Rovira-Clavé, Alexandros P Drainas, Sizun Jiang, Yunhao Bai, Maya Baron, Bokai Zhu, Alec E Dallas, Myung Chang Lee, Theresa P Chu, Alessandra Holzem and 10 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer cell, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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  18. Highly Multiplexed Immunofluorescence Imaging of Mouse Oocytes.Methods in molecular biology (Clifton, N.J.) · 2025
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Xavier Rovira-ClavéDepartment of Pathology, Stanford University, Stanford, CA 94305, USA; Department of Microbiology and Immunology, Stanford University, Stanford, CA 94305, USA.
Alexandros P DrainasDepartment of Pediatrics, Stanford University, Stanford, CA 94305, USA; Department of Genetics, Stanford University, Stanford, CA 94305, USA.
Sizun JiangDepartment of Pathology, Stanford University, Stanford, CA 94305, USA.
Yunhao BaiDepartment of Pathology, Stanford University, Stanford, CA 94305, USA.
Maya BaronDepartment of Pediatrics, Stanford University, Stanford, CA 94305, USA; Department of Genetics, Stanford University, Stanford, CA 94305, USA.
Bokai ZhuDepartment of Pathology, Stanford University, Stanford, CA 94305, USA; Department of Microbiology and Immunology, Stanford University, Stanford, CA 94305, USA.
Alec E DallasDepartment of Pediatrics, Stanford University, Stanford, CA 94305, USA; Department of Genetics, Stanford University, Stanford, CA 94305, USA.
Myung Chang LeeDepartment of Pediatrics, Stanford University, Stanford, CA 94305, USA; Department of Genetics, Stanford University, Stanford, CA 94305, USA.
Theresa P ChuDepartment of Pathology, Stanford University, Stanford, CA 94305, USA.
Alessandra HolzemDepartment of Pediatrics, Stanford University, Stanford, CA 94305, USA; Department of Genetics, Stanford University, Stanford, CA 94305, USA.
Ramya AyyagariDepartment of Pediatrics, Stanford University, Stanford, CA 94305, USA; Department of Genetics, Stanford University, Stanford, CA 94305, USA.
Debadrita BhattacharyaDepartment of Pediatrics, Stanford University, Stanford, CA 94305, USA; Department of Genetics, Stanford University, Stanford, CA 94305, USA.
Erin F McCaffreyDepartment of Pathology, Stanford University, Stanford, CA 94305, USA.
Noah F GreenwaldDepartment of Pathology, Stanford University, Stanford, CA 94305, USA.
Maxim MarkovicDepartment of Pathology, Stanford University, Stanford, CA 94305, USA.
Garry L ColesDepartment of Pediatrics, Stanford University, Stanford, CA 94305, USA; Department of Genetics, Stanford University, Stanford, CA 94305, USA.
Michael AngeloDepartment of Pathology, Stanford University, Stanford, CA 94305, USA.
Michael C BassikDepartment of Genetics, Stanford University, Stanford, CA 94305, USA.
Julien SageDepartment of Pediatrics, Stanford University, Stanford, CA 94305, USA; Department of Genetics, Stanford University, Stanford, CA 94305, USA. Electronic address: julsage@stanford.edu.
Garry P NolanDepartment of Pathology, Stanford University, Stanford, CA 94305, USA. Electronic address: gnolan@stanford.edu.

Funding

Using a tonsil organoid system to probe conditions for the induction of protective antibody and T cell responses to influenza.U19AI057229 · NIAID · STANFORD UNIVERSITY · PI Mark Morris Davis · 2003 to 2026
$88.5M
Systems Biology CoreU19AI100627 · NIAID · SCRIPPS RESEARCH INSTITUTE, THE · PI NOLAN, GARRY P · 2012 to 2021
$49.1M
The Cellular Geography of Therapeutic Resistance in CancerU2CCA233195 · NCI · DANA-FARBER CANCER INST · PI VAN ALLEN, ELIEZER M · 2018 to 2023
$13.7M
Spatial-Genomic Integrative Multi-Species Analysis of Lymph Node MetastasisU54CA209971 · NCI · STANFORD UNIVERSITY · PI NOLAN, GARRY P, PLEVRITIS, SYLVIA KATINA · 2016 to 2021
$11.0M
Single-Cell Biology and Data Analysis Shared Resource (SCB-DA SR)U54CA217450 · NCI · VANDERBILT UNIVERSITY · PI WEAVER, ALISSA M · 2018 to 2022
$8.5M
The Lung PCA: A Multi-Dimensional Atlas of Pulmonary PremalignancyU2CCA233238 · NCI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI DUBINETT, STEVEN M., SPIRA, AVRUM E · 2018 to 2021
$7.0M
Investigating molecular and cellular mechanisms of SCLC development to identify novel therapeutic strategiesR35CA231997 · NCI · STANFORD UNIVERSITY · PI SAGE, JULIEN · 2019 to 2025
$6.2M
Stanford Tissue Mapping CenterU54HG010426 · NHGRI · STANFORD UNIVERSITY · PI PLEVRITIS, SYLVIA KATINA · 2018 to 2021
$5.1M
Spatial-Temporal Dissection of Stratified Host Tissue Responses to Severe acute respiratory syndrome-related coronaviruses in situ to Understand Intra-host PathogenesisDP2AI171139 · NIAID · BETH ISRAEL DEACONESS MEDICAL CENTER · PI Sizun Jiang · 2022 to 2026
$2.3M
Cancer Research UK 27145NCI NIH HHS R35 CA231997NCI NIH HHS U2C CA233195NCI NIH HHS U2C CA233238NCI NIH HHS U54 CA209971NCI NIH HHS U54 CA217450NHGRI NIH HHS U54 HG010426NIAID NIH HHS DP2 AI171139NIAID NIH HHS U19 AI057229NIAID NIH HHS U19 AI100627
6 · The paper itself

Abstract

Intratumoral heterogeneity is a seminal feature of human tumors contributing to tumor progression and response to treatment. Current technologies are still largely unsuitable to accurately track phenotypes and clonal evolution within tumors, especially in response to genetic manipulations. Here, we developed epitopes for imaging using combinatorial tagging (EpicTags), which we coupled to multiplexed ion beam imaging (EpicMIBI) for in situ tracking of barcodes within tissue microenvironments. Using EpicMIBI, we dissected the spatial component of cell lineages and phenotypes in xenograft models of small cell lung cancer. We observed emergent properties from mixed clones leading to the preferential expansion of clonal patches for both neuroendocrine and non-neuroendocrine cancer cell states in these models. In a tumor model harboring a fraction of PTEN-deficient cancer cells, we observed a non-autonomous increase of clonal patch size in PTEN wild-type cancer cells. EpicMIBI facilitates in situ interrogation of cell-intrinsic and cell-extrinsic processes involved in intratumoral heterogeneity.

Indexed as

NeoplasmsCell LineageClonal EvolutionClone CellsEpitopesHumansTumor MicroenvironmentEpitopesMIBImultiplex imagingneuroendocrinePTENSCLCspatial barcodingtumor heterogeneity

Identifiers

PMID36240778
PMCPMC9673683

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.