ArticleMolecules (Basel, Switzerland)2022
The Development and Application of a HPTLC-Derived Database for the Identification of Phenolics in Honey.
Article in Molecules (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Recent Progress on Polyphenols of Malaysian Honey and Their Molecular Mechanism Pathways in Cancer-A Comprehensive Review.International journal of molecular sciences · 2026Review
- Comprehensive Metabolite Profiling and Enzyme Inhibitory Potential of Psidium guajava L. Hydro Alcoholic Extract: An In vitro and In Silico Approach.Chemistry & biodiversity · 2026Article
- PLANTA Protocol for the Direct Detection and Identification of Bioactive Compounds in Complex Mixtures via Combined NMR-HPTLC-Based Heterocovariance.Analytical chemistry · 2025Article
- Phytochemical Profile and Biological Activities of Rtanj'sPlants (Basel, Switzerland) · 2025Article
- Physicochemical Characteristics, Antioxidant Properties, and Identification of Bioactive Compounds in Australian Stingless Bee Honey Using High-Performance Thin-Layer Chromatography.Molecules (Basel, Switzerland) · 2025Article
- The comparison of the antioxidant, antibacterial and antiviral potential of Polish fir honeydew and Manuka honeys.Scientific reports · 2024Article
- HPTLC Combined with sHetCA and Multivariate Statistics for the Detection of Bioactive Compounds in Complex Mixtures.Molecules (Basel, Switzerland) · 2024Article
- Physicochemical Properties, Antioxidant Activity, and High-Performance Thin-Layer Chromatography Profiling of Propolis Samples from Western Australia.Plants (Basel, Switzerland) · 2024Article
- Estrogenic Isoflavones in Clover Plants, Flower Nectar, Unripe Honeys and Mature Honeys: A Natural Biochemical Transformation of Isoflavones by Honeybees.Foods (Basel, Switzerland) · 2024Article
- Antioxidant Activity and Phenolic Compound Identification and Quantification in Western Australian Honeys.Antioxidants (Basel, Switzerland) · 2023Article
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Authors and funding
5 authors.
Funding
Abstract
This study reports on the development and validation of a HPTLC-derived database to identify phenolic compounds in honey. Two database sets are developed to contain the profiles of 107 standard compounds. Rich data in the form of Rf values, colour hues (H°) at 254 nm and 366 nm, at 366 nm after derivatising with natural product PEG reagent, and at 366 nm and white light after derivatising with vanillin-sulfuric acid reagent, λ max and λ min values in their fluorescence and λ max values in their UV-Vis spectra as well as λ max values in their fluorescence and UV-Vis spectra after derivatisation are used as filtering parameters to identify potential matches in a honey sample. A spectral overlay system is also developed to confirm these matches. The adopted filtering approach is used to validate the database application using positive and negative controls and also by comparing matches with those identified via HPLC-DAD. Manuka honey is used as the test honey and leptosperine, mandelic acid, kojic acid, lepteridine, gallic acid, epigallocatechin gallate, 2,3,4-trihydroxybenzoic acid, o-anisic acid and methyl syringate are identified in the honey using the HPTLC-derived database.
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