Evidence map›Paper›PMID 36234919›Full record

ArticleMolecules (Basel, Switzerland)2022

High Endogenously Synthesized N-3 Polyunsaturated Fatty Acids in Fat-1 Mice Attenuate High-Fat Diet-Induced Insulin Resistance by Inhibiting NLRP3 Inflammasome Activation via Akt/GSK-3β/TXNIP Pathway.

Pan Zhu, Jin-Jie Zhang, Yi Cen, Yong Yang, Feng Wang, Kun-Peng Gu, Hai-Tao Yang, Yun-Zhi Wang, Zu-Quan Zou

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 21 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 2 countries.

Pan ZhuNingbo Beilun District for Disease Control and Prevention, Ningbo 315000, China.
Jin-Jie ZhangCollege of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, China.
Yi CenCollege of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, China.
Yong YangDepartment of Clinical Laboratory, The Affiliated Hospital of Ningbo University, Ningbo 315020, China.
Feng WangDepartment of Laboratory Medicine, Ningbo Medical Center Lihuili Hospital, Ningbo 315048, China.
Kun-Peng GuNingbo Beilun District for Disease Control and Prevention, Ningbo 315000, China.
Hai-Tao YangDepartment of Pathology, Mingzhou Hospital of Zhejiang University, Ningbo 315199, China.
Yun-Zhi WangSchool of Health Sciences, University of Sydney, P.O. Box 170, Lidcombe, Sydney, NSW 1825, Australia.
Zu-Quan ZouNingbo Beilun District for Disease Control and Prevention, Ningbo 315000, China.ORCID 0000-0002-9247-4734
Ningbo University · CNNingbo Center for Disease Control and Prevention · CNNingbo Medical Center Lihuili Hospital · CNThe University of Sydney · AUZhejiang University · CN

Funding

Clinical Research Fund of Zhejiang Medical Association 2019ZYC-A112National Key R&D Program of China 2018YFD0901105Zhejiang Key Laboratory of Pathophysiology 201802Zhejiang Province Public Welfare Technology Application Research Project LGN19C200010；LGN20C200013
6 · The paper itself

Abstract

High-fat (HF) diets and low-grade chronic inflammation contribute to the development of insulin resistance and type 2 diabetes (T2D), whereas n-3 polyunsaturated fatty acids (PUFAs), due to their anti-inflammatory effects, protect against insulin resistance. Interleukin (IL)-1β is implicated in insulin resistance, yet how n-3 PUFAs modulate IL-1β secretion and attenuate HF diet-induced insulin resistance remains elusive. In this study, a HF diet activated NLRP3 inflammasome via inducing reactive oxygen species (ROS) generation and promoted IL-1β production primarily from adipose tissue preadipocytes, but not from adipocytes and induced insulin resistance in wild type (WT) mice. Interestingly, endogenous synthesized n-3 polyunsaturated fatty acids (PUFAs) reversed this process in HF diet-fed fat-1 transgenic mice although the HF diet induced higher weight gain in fat-1 mice, compared with the control diet. Mechanistically, palmitic acid (PA), the main saturated fatty acid in an HF diet inactivated AMPK and led to decreased GSK-3β phosphorylation, at least partially through reducing Akt activity, which ultimately blocked the Nrf2/Trx1 antioxidant pathway and induced TXNIP cytoplasm translocation and NLRP3 inflammasome activation, whereas docosahexaenoic acid (DHA), the most abundant n-3 PUFA in fat-1 adipose tissue, reversed this process via inducing Akt activation. Our

Indexed as

Diabetes Mellitus, Type 2Fatty Acids, Omega-3Insulin ResistanceAMP-Activated Protein KinasesAnimalsAnti-Inflammatory AgentsAntioxidantsCarrier ProteinsDiet, High-FatDocosahexaenoic AcidsFatty AcidsGlycogen Synthase Kinase 3 betaInflammasomesMiceMice, Inbred C57BLNF-E2-Related Factor 2AMP-Activated Protein KinasesAnti-Inflammatory AgentsAntioxidantsCarrier ProteinsDocosahexaenoic AcidsFatty AcidsFatty Acids, Omega-3Glycogen Synthase Kinase 3 betaInflammasomesNF-E2-Related Factor 2NLR Family, Pyrin Domain-Containing 3 ProteinNlrp3 protein, mousePalmitic AcidProto-Oncogene Proteins c-aktReactive Oxygen SpeciesRNA, Small InterferingThioredoxinsTxnip protein, mousedocosahexaenoic acidfat-1IL-1βinsulin resistancen-3 PUFAsNLRP3 inflammasomeobesity

Identifiers

PMID36234919
PMCPMC9570616
OpenAlexW4297546461

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.