Evidence map›Paper›PMID 36233334›Full record

ArticleInternational journal of molecular sciences2022

Interactions between C8orf37 and FAM161A, Two Ciliary Proteins Essential for Photoreceptor Survival.

Yu Liu, Jinjun Chen, Rachel Sager, Erika Sasaki, Huaiyu Hu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 56% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 3 countries.

Yu LiuCenter for Vision Research, Departments of Neuroscience and Physiology and of Ophthalmology and Visual Sciences, Upstate Medical University, Syracuse, NY 13210, USA.
Jinjun ChenCenter for Vision Research, Departments of Neuroscience and Physiology and of Ophthalmology and Visual Sciences, Upstate Medical University, Syracuse, NY 13210, USA.
Rachel SagerCenter for Vision Research, Departments of Neuroscience and Physiology and of Ophthalmology and Visual Sciences, Upstate Medical University, Syracuse, NY 13210, USA.
Erika SasakiDepartment of Marmoset Biology and Medicine, Central Institute for Experimental Animals, Tonomachi, Kawasaki 210-0821, Kanagawa, Japan.ORCID 0000-0002-0415-5400
Huaiyu HuCenter for Vision Research, Departments of Neuroscience and Physiology and of Ophthalmology and Visual Sciences, Upstate Medical University, Syracuse, NY 13210, USA.
SUNY Upstate Medical University · USCentral Institute for Experimental Animals · JP

Funding

Mechanisms of cognitive deficits in dystroglycanopathiesR01HD083680 · NICHD · UPSTATE MEDICAL UNIVERSITY · PI HU, HUAIYU · 2015 to 2019
$2.8M
Ciliary pcoket matrix in photoreceptor healthR01EY028555 · NEI · UPSTATE MEDICAL UNIVERSITY · PI HU, HUAIYU · 2018 to 2022
$2.0M
The Roles of EYS in photoreceptor healthR21EY031002 · NEI · UPSTATE MEDICAL UNIVERSITY · PI HU, HUAIYU · 2020 to 2021
$439k
NEI NIH HHS R01 EY028555NEI NIH HHS R21 EY031002NICHD NIH HHS R01 HD083680NIH HHS R01EY028555, R21EY031002
6 · The paper itself

Abstract

Mutations in C8orf37 cause Bardet-Biedl syndrome (BBS), retinitis pigmentosa (RP), and cone-rod dystrophy (CRD), all manifest in photoreceptor degeneration. Little is known about which proteins C8orf37 interacts with to contribute to photoreceptor survival. To determine the proteins that potentially interact with C8orf37, we carried out a yeast two-hybrid (Y2H) screen using C8orf37 as a bait. FAM161A, a microtubule-binding protein localized at the photoreceptor cilium required for photoreceptor survival, was identified as one of the preys. Double immunofluorescence staining and proximity ligation assay (PLA) of marmoset retinal sections showed that C8orf37 was enriched and was co-localized with FAM161A at the ciliary base of photoreceptors. Epitope-tagged C8orf37 and FAM161A, expressed in HEK293 cells, were also found to be co-localized by double immunofluorescence staining and PLA. Furthermore, interaction domain mapping assays identified that the N-terminal region of C8orf37 and amino acid residues 341-517 within the PFAM UPF0564 domain of FAM161A were critical for C8orf37-FAM161A interaction. These data suggest that the two photoreceptor survival proteins, C8orf37 and FAM161A, interact with each other which may contribute to photoreceptor health.

Indexed as

Eye ProteinsProteinsRetinitis PigmentosaAmino AcidsEpitopesHEK293 CellsHumansPhotoreceptor CellsAmino AcidsCFAP418 protein, humanEpitopesEye ProteinsFAM161A protein, humanProteinsBardet-Biedl syndromeC8orf37cone–rod dystrophyFAM161Aretinal degenerationretinitis pigmentosa

Identifiers

PMID36233334
PMCPMC9570145
OpenAlexW4304136602

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.