Evidence map›Paper›PMID 36232839›Full record

ArticleInternational journal of molecular sciences2022

Enzymatic Construction of DARPin-Based Targeted Delivery Systems Using Protein Farnesyltransferase and a Capture and Release Strategy.

Yi Zhang, Yiao Wang, Safak Uslu, Sneha Venkatachalapathy, Mohammad Rashidian, Jonas V Schaefer, Andreas Plückthun, Mark D Distefano

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Introduction of Carbonyl Groups into Antibodies.Molecules (Basel, Switzerland) · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Yi ZhangDepartment of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.
Yiao WangDepartment of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.
Safak UsluDepartment of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, MA 02215, USA.
Sneha VenkatachalapathyDepartment of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.ORCID 0000-0002-6257-8874
Mohammad RashidianDepartment of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Boston, MA 02215, USA.
Jonas V SchaeferDepartment of Biochemistry, University of Zurich, 8057 Zurich, Switzerland.
Andreas PlückthunDepartment of Biochemistry, University of Zurich, 8057 Zurich, Switzerland.ORCID 0000-0003-4191-5306
Mark D DistefanoDepartment of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.ORCID 0000-0002-2872-0259
University of Minnesota · USDana-Farber Cancer Institute · USUniversity of Zurich · CH

Funding

Enzymatic Protein LabelingR01GM084152 · NIGMS · UNIVERSITY OF MINNESOTA · PI DISTEFANO, MARK D · 2010 to 2019
$2.6M
Chemical Approaches for Exploring Protein Prenylation in Living CellsR35GM141853 · NIGMS · UNIVERSITY OF MINNESOTA · PI MARK D DISTEFANO · 2021 to 2026
$2.4M
NIGMS NIH HHS R01 GM084152NIGMS NIH HHS R35 GM141853NIH HHS R01 GM084152NIH HHS R35 GM141853
6 · The paper itself

Abstract

Protein-based conjugates have been extensively utilized in various biotechnological and therapeutic applications. In order to prepare homogeneous conjugates, site-specific modification methods and efficient purification strategies are both critical factors to be considered. The development of general and facile conjugation and purification strategies is therefore highly desirable. Here, we apply a capture and release strategy to create protein conjugates based on Designed Ankyrin Repeat Proteins (DARPins), which are engineered antigen-binding proteins with prominent affinity and selectivity. In this case, DARPins that target the epithelial cell adhesion molecule (EpCAM), a diagnostic cell surface marker for many types of cancer, were employed. The DARPins were first genetically modified with a C-terminal CVIA sequence to install an enzyme recognition site and then labeled with an aldehyde functional group employing protein farnesyltransferase. Using a capture and release strategy, conjugation of the labeled DARPins to a TAMRA fluorophore was achieved with either purified proteins or directly from crude

Indexed as

Ankyrin RepeatDesigned Ankyrin Repeat ProteinsAldehydesAlkyl and Aryl TransferasesEpithelial Cell Adhesion MoleculeEscherichia coliHumansProteinsAldehydesAlkyl and Aryl TransferasesDesigned Ankyrin Repeat ProteinsEpithelial Cell Adhesion Moleculep21(ras) farnesyl-protein transferaseProteinsbiorthogonal conjugationprotein farnesyltransferasesite-specific protein conjugationtargeted drug delivery

Identifiers

PMID36232839
PMCPMC9569580
OpenAlexW4297982321

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.