ArticleCells2022
Morphology of Phagophore Precursors by Correlative Light-Electron Microscopy.
Article in Cells, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Internalized components of membrane attack complexes disrupt proteostasis and acquire alarmin-like properties.Nature communications · 2026Article
- Internalized Components of Membrane Attack Complexes Disrupt Proteostasis and Acquire Alarmin-Like Properties.bioRxiv : the preprint server for biology · 2026Article
- Where and how do mammalian cells shape autophagosomes?The Journal of biological chemistry · 2026Review
- Cutting edge: ESCRT-mediated phagophore closure in mammals.The Biochemical journal · 2026Review
- Regulation of intracellular proliferation of Salmonella typhimurium by CD63.Genes & genomics · 2025Article
- Biogenesis of omegasomes and autophagosomes in mammalian autophagy.Biochemical Society transactions · 2024Review
- Failure of Autophagy in Pompe Disease.Biomolecules · 2024Review
- Membrane association of the ATG8 conjugation machinery emerges as a key regulatory feature for autophagosome biogenesis.FEBS letters · 2024Review
- Growing thin - How bulk lipid transport drives expansion of the autophagosome membrane but not of its lumen.Current opinion in cell biology · 2023Review
- ATG9 vesicles comprise the seed membrane of mammalian autophagosomes.The Journal of cell biology · 2023Article
- Review
- Autophagosome Biogenesis.Cells · 2023Review
- Improved volume CLEM revealed that aberrant phagophores and RB1CC1/FIP200-containing clusters appear surround SQSTM1/p62 aggregates inAutophagy reports · 2023Article
- Mitophagy in ototoxicity.Frontiers in cellular neuroscience · 2023Review
- Quantitative 3D correlative light and electron microscopy of organelle association during autophagy.Cell structure and function · 2022Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
Abstract
Autophagosome biogenesis occurs in the transient subdomains of the endoplasmic reticulum that are called omegasomes, which, in fluorescence microscopy, appear as small puncta, which then grow in diameter and finally shrink and disappear once the autophagosome is complete. Autophagosomes are formed by phagophores, which are membrane cisterns that elongate and close to form the double membrane that limits autophagosomes. Earlier electron-microscopy studies showed that, during elongation, phagophores are lined by the endoplasmic reticulum on both sides. However, the morphology of the very early phagophore precursors has not been studied at the electron-microscopy level. We used live-cell imaging of cells expressing markers of phagophore biogenesis combined with correlative light-electron microscopy, as well as electron tomography of ATG2A/B-double-deficient cells, to reveal the high-resolution morphology of phagophore precursors in three dimensions. We showed that phagophores are closed or nearly closed into autophagosomes already at the stage when the omegasome diameter is still large. We further observed that phagophore precursors emerge next to the endoplasmic reticulum as bud-like highly curved membrane cisterns with a small opening to the cytosol. The phagophore precursors then open to form more flat cisterns that elongate and curve to form the classically described crescent-shaped phagophores.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.