Evidence map›Paper›PMID 36230920›Full record

ArticleCells2022

Timing of Blood Sample Processing Affects the Transcriptomic and Epigenomic Profiles in CD4

Fahd Alhamdan, Kristina Laubhahn, Christine Happle, Anika Habener, Adan C Jirmo, Clemens Thölken, Raffaele Conca, Ho-Ryun Chung, Gesine Hansen, Daniel P Potaczek and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 2 countries.

Fahd AlhamdanTranslational Inflammation Research Division & Core Facility for Single Cell Multiomics, Medical Faculty, Philipps University of Marburg, Member of the German Center for Lung Research (DZL) and the Universities of Giessen and Marburg Lung Center, D-35043 Marburg, Germany.
Kristina LaubhahnPediatric Allergology, Department of Pediatrics, Dr. von Hauner Children's Hospital, University Hospital, Ludwig Maximilian University (LMU) of Munich, Comprehensive Pneumology Center Munich (CPC-M), Member of the German Center for Lung Research (DZL), D-80337 Munich, Germany.
Christine HappleDepartment of Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL) and the Cluster of Excellence RESIST (EXC 2155), D-30625 Hannover, Germany.
Anika HabenerDepartment of Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL) and the Cluster of Excellence RESIST (EXC 2155), D-30625 Hannover, Germany.
Adan C JirmoDepartment of Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL) and the Cluster of Excellence RESIST (EXC 2155), D-30625 Hannover, Germany.ORCID 0000-0002-4693-0081
Clemens ThölkenInstitute of Medical Bioinformatics and Biostatistics, Medical Faculty, Philipps University of Marburg, D-35032 Marburg, Germany.ORCID 0000-0003-4328-9193
Raffaele ConcaPediatric Allergology, Department of Pediatrics, Dr. von Hauner Children's Hospital, University Hospital, Ludwig Maximilian University (LMU) of Munich, Comprehensive Pneumology Center Munich (CPC-M), Member of the German Center for Lung Research (DZL), D-80337 Munich, Germany.
Ho-Ryun ChungInstitute of Medical Bioinformatics and Biostatistics, Medical Faculty, Philipps University of Marburg, D-35032 Marburg, Germany.
Gesine HansenDepartment of Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL) and the Cluster of Excellence RESIST (EXC 2155), D-30625 Hannover, Germany.
Daniel P PotaczekTranslational Inflammation Research Division & Core Facility for Single Cell Multiomics, Medical Faculty, Philipps University of Marburg, Member of the German Center for Lung Research (DZL) and the Universities of Giessen and Marburg Lung Center, D-35043 Marburg, Germany.ORCID 0000-0003-0324-8506
Bianca SchaubPediatric Allergology, Department of Pediatrics, Dr. von Hauner Children's Hospital, University Hospital, Ludwig Maximilian University (LMU) of Munich, Comprehensive Pneumology Center Munich (CPC-M), Member of the German Center for Lung Research (DZL), D-80337 Munich, Germany.ORCID 0000-0003-1652-8873
Ruth GrychtolDepartment of Pediatric Pneumology, Allergology and Neonatology, Hannover Medical School, Biomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), Member of the German Center for Lung Research (DZL) and the Cluster of Excellence RESIST (EXC 2155), D-30625 Hannover, Germany.
Holger GarnTranslational Inflammation Research Division & Core Facility for Single Cell Multiomics, Medical Faculty, Philipps University of Marburg, Member of the German Center for Lung Research (DZL) and the Universities of Giessen and Marburg Lung Center, D-35043 Marburg, Germany.ORCID 0000-0002-5178-4023
Medizinische Hochschule Hannover · DEPhilipps University of Marburg · DEGerman Center for Lung Research · DEBrigham and Women's Hospital · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Optimal pre-analytical conditions for blood sample processing and isolation of selected cell populations for subsequent transcriptomic and epigenomic studies are required to obtain robust and reproducible results. This pilot study was conducted to investigate the potential effects of timing of CD4

Indexed as

EpigenomicsTranscriptomeAdultCD4-Positive T-LymphocytesHistonesHumansPilot ProjectsSpecimen HandlingT-LymphocytesHistonesCD4+ T-cellsChIP sequencingepigeneticshistone modificationstranscriptomics

Identifiers

PMID36230920
PMCPMC9563434
OpenAlexW4296848456

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.