ArticleAnimals : an open access journal from MDPI2022
The Effect of Different Extenders on the Quality Characteristics of European Red Deer Epididymal Sperm Stored at 5 °C.
Article in Animals : an open access journal from MDPI, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
7 citing papers in PubMed.
- Enriching egg yolk fractions-tris-based extenders with cow skimmed milk improved the cryopreservation of bull sperm.Veterinary research forum : an international quarterly journal · 2026Article
- Developing a simple universal hypo-osmotic swelling test (HOST) for assessing sperm membrane integrity in pigs, rabbits, and goats.Journal of advanced veterinary and animal research · 2025Article
- Improvement of Post-Thaw Quality and In Vivo Fertility of Simmental Bull Spermatozoa Using Ferulic Acid.Veterinary medicine and science · 2024Article
- Changes in the Morphology and Antioxidant Status of European Red Deer Sperm Stored in the Epididymides and in a Liquid State.Animals : an open access journal from MDPI · 2024Article
- β-Nicotinamide mononucleotide improves chilled ram sperm quality in vitro by reducing oxidative stress damage.Animal bioscience · 2024Article
- Fluorescence Microscopy and Flow-Cytometry Assessment of Substructures in European Red Deer Epididymal Spermatozoa after Cryopreservation.Animals : an open access journal from MDPI · 2023Article
- Current Status and Advances in Semen Preservation.Animals : an open access journal from MDPI · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The aim of this study was to evaluate the effect of different extenders on the quality of European red deer epididymal sperm stored at 5 °C. Epididymal spermatozoa were collected post mortem from 10 stags and diluted with three extenders (Bovidyl®, BoviFree®, and BioXcell®) and stored at 5 °C. Sperm motility (TMOT), motility parameters (system CASA), plasma membrane integrity (SYBR-14+/PI−), acrosomal membrane integrity (FITC-PNA−/PI−), mitochondrial activity (JC-1/PI), viability, and apoptotic-like changes (YOPRO/PI) were evaluated. The analyses were conducted on the first and successive days of storage (D1−D7). The applied extender, storage time, and the interactions between these factors significantly (p < 0.001) affected most of the analyzed parameters whose values were highest in sperm samples stored in Bovidyl®, regardless of storage time. In Bovidyl®, BoviFree®, and BioXcell® extenders, TMOT values were estimated at 83%, 63%, and 59%, respectively, on D3. The extenders significantly influenced DNA integrity on D7. The percentage of dead sperm increased from D3. The quality of stored sperm cells was significantly influenced by the extenders’ biochemical composition. BoviFree® and BioXcell® contain glycerol which could contribute to deteriorating the quality of spermatozoa stored at 5 °C. Sperm cells stored in the egg yolk-based extender (Bovidyl®) were characterized by the highest viability and functionality.
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Registered trials
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