ArticleJournal of biological engineering2022
DC electrical stimulation enhances proliferation and differentiation on N2a and MC3T3 cell lines.
Article in Journal of biological engineering, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 33 citations in OpenAlex.
- Bystander Effect Induced by Electrical Stimulation Promotes N2a Differentiation Through Interleukin-6.Biosensors · 2026Article
- Bone Growth Stimulators to Enhance Spinal Fusion Outcomes.Spine surgery and related research · 2026Review
- Electrical Microenvironment Reconstruction and the Application of Biomaterials in Spinal Cord Injury.Journal of functional biomaterials · 2026Review
- In vitro electrical stimulation devices: practical framework for design, fabrication, and operation.Journal of biological engineering · 2026Article
- Magnetically Responsive Piezoelectric Nanocapacitors Enhance Neural Recovery Following Spinal Cord Injury via Targeted Spinal Magnetic Stimulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Electrical stimulation combined with p27Kip1 inactivation drives proliferative neurogenic reprogramming of Mueller glia in the adult mouse retina.bioRxiv : the preprint server for biology · 2026Article
- Alternating Current Stimulation Promotes Healing of Bone Fractures in Rabbits.Bioengineering (Basel, Switzerland) · 2025Article
- Schottky engineering of GDYO@Pt to boost piezoelectric and oxidative stress modulation for accelerated cranial regeneration.Nature communications · 2025Article
- Advanced therapeutic scaffolds of biomimetic periosteum for functional bone regeneration.Journal of nanobiotechnology · 2025Review
- Accelerated Bone Healing via Electrical Stimulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Formation of Neurointerfaces Based on Electrically Conductive Biopolymers by Two-Photon Polymerization Method.Polymers · 2025Article
- Direct Current Stimulation (DCS) Modulates Lipid Metabolism and Intercellular Vesicular Trafficking in SHSY-5Y Cell Line: Implications for Parkinson's Disease.Journal of neurochemistry · 2025Article
- Agarose hydrogel-mediated electroporation method for retinal tissue cultured at the air-liquid interface.iScience · 2024Article
- Advances in electroactive biomaterials: Through the lens of electrical stimulation promoting bone regeneration strategy.Journal of orthopaedic translation · 2024Review
- Electrical pulse stimulation parameters modulate N2a neuronal differentiation.Cell death discovery · 2024Article
- Evaluation of clinical and imaging features for differentiating rhabdomyosarcoma from neuroblastoma in pediatric soft tissue.Frontiers in oncology · 2024Article
- Discrimination between the effects of pulsed electrical stimulation and electrochemically conditioned medium on human osteoblasts.Journal of biological engineering · 2023Article
- Impact of exogenous direct electric current on the expression of mRNA related to OPG, in SaOS-2 cells using quantitative reverse transcription polymerase chain reaction: A qualitative and quantitative analysis.Journal of oral biology and craniofacial researchArticle
- Optimizing Alternating Current Electrical Stimulation Parameters to Enhance Osteoblasts Differentiation.BioFactors (Oxford, England)Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundElectrical stimulation is a novel tool to promote the differentiation and proliferation of precursor cells. In this work we have studied the effects of direct current (DC) electrical stimulation on neuroblastoma (N2a) and osteoblast (MC3T3) cell lines as a model for nervous and bone tissue regeneration, respectively. We have developed the electronics and encapsulation of a proposed stimulation system and designed a setup and protocol to stimulate cell cultures.
methodsCell cultures were subjected to several assays to assess the effects of electrical stimulation on them. N2a cells were analyzed using microscope images and an inmunofluorescence assay, differentiated cells were counted and neurites were measured. MC3T3 cells were subjected to an AlamarBlue assay for viability, ALP activity was measured, and a real time PCR was carried out.
resultsOur results show that electrically stimulated cells had more tendency to differentiate in both cell lines when compared to non-stimulated cultures, paired with a promotion of neurite growth and polarization in N2a cells and an increase in proliferation in MC3T3 cell line.
conclusionsThese results prove the effectiveness of electrical stimulation as a tool for tissue engineering and regenerative medicine, both for neural and bone injuries. Bone progenitor cells submitted to electrical stimulation have a higher tendency to differentiate and proliferate, filling the gaps present in injuries. On the other hand, neuronal progenitor cells differentiate, and their neurites can be polarized to follow the electric field applied.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.