Evidence map›Paper›PMID 36228757›Full record

ArticleToxicon : official journal of the International Society on Toxinology2022

Venom characterization of the Brazilian Pampa snake Bothrops pubescens by top-down and bottom-up proteomics.

Darlene Lopes Rangel, Rafael D Melani, Evelise Leis Carvalho, Juliano Tomazzoni Boldo, Tiago Gomes Dos Santos, Neil L Kelleher, Paulo Marcos Pinto

Abstract read
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In one paragraph

Article in Toxicon : official journal of the International Society on Toxinology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Venomics across theJournal of proteome research · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Darlene Lopes RangelApplied Proteomics Laboratory, Federal University of Pampa, São Gabriel, Brazil; Pos Graduation in Biological Sciences, Federal University of Pampa, São Gabriel, Brazil.
Rafael D MelaniDepartments of Molecular Biosciences, Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, 60208, United States.
Evelise Leis CarvalhoApplied Proteomics Laboratory, Federal University of Pampa, São Gabriel, Brazil; Pos Graduation in Biological Sciences, Federal University of Pampa, São Gabriel, Brazil.
Juliano Tomazzoni BoldoPos Graduation in Biological Sciences, Federal University of Pampa, São Gabriel, Brazil.
Tiago Gomes Dos SantosPampa Biodiversity Studies Laboratory (LEBIP), Federal University of Pampa, São Gabriel, Brazil.
Neil L KelleherDepartments of Molecular Biosciences, Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, 60208, United States.
Paulo Marcos PintoApplied Proteomics Laboratory, Federal University of Pampa, São Gabriel, Brazil; Pos Graduation in Biological Sciences, Federal University of Pampa, São Gabriel, Brazil. Electronic address: paulopinto@unipampa.edu.br.

Funding

TR&D 7: Cell Specific ProteomicsP41GM108569 · NIGMS · NORTHWESTERN UNIVERSITY · PI KELLEHER, NEIL L · 2015 to 2024
$13.6M
Acquisition of a Thermo Fisher Scientific Q Exactive UHMR mass spectrometerS10OD025194 · OD · NORTHWESTERN UNIVERSITY · PI COMPTON, PHILIP DANIEL · 2018 to 2018
$795k
NIGMS NIH HHS P41 GM108569NIH HHS S10 OD025194
6 · The paper itself

Abstract

The envenomation from the Bothrops genus is characterized by systemic and local effects caused by the main toxin families in the venom. In Bothrops pubescens venom we were able to identify 89 protein groups belonging to 13 toxin families with the bottom-up proteomics approach and 40 unique proteoforms belonging to 6 toxin families with the top-down proteomics approach. We also identified multi-proteoform complexes of dimeric L-amino acid oxidase using native top-down mass spectrometry.

Indexed as

BothropsCrotalid VenomsAnimalsBrazilMass SpectrometryProteomeProteomicsCrotalid VenomsProteomeBothropsEnvenomationL-amino acid OxidaseVenomics

Identifiers

PMID36228757
PMCPMC9901210

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.