Evidence map›Paper›PMID 36227560›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Characterizing Fluorescence Properties of Turn-on RNA Aptamers.

Robert J Trachman, Katie A Link, Jay R Knutson, Adrian R Ferré-D'Amaré

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Robert J TrachmanBiochemistry and Biophysics Center, National Heart, Lung and Blood Institute, Bethesda, MD, USA.
Katie A LinkBiochemistry and Biophysics Center, National Heart, Lung and Blood Institute, Bethesda, MD, USA.
Jay R KnutsonBiochemistry and Biophysics Center, National Heart, Lung and Blood Institute, Bethesda, MD, USA.
Adrian R Ferré-D'AmaréBiochemistry and Biophysics Center, National Heart, Lung and Blood Institute, Bethesda, MD, USA. adrian.ferre@nih.gov.

Funding

RNA: Structure, Biophysics and PhysiologyZIAHL006102 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI FERRÉ-D'AMARÉ, ADRIAN · 2011 to 2025
$14.6M
RNA: Tools for Cell BiologyZIAHL006188 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI FERRÉ-D'AMARÉ, ADRIAN · 2014 to 2025
$9.5M
Time Resolved Fluorescence SpectroscopyZIAHL001452 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI KNUTSON, JAY R · 2009 to 2025
$5.1M
Intramural NIH HHS Z99 HL999999Intramural NIH HHS ZIA HL006102Intramural NIH HHS ZIA HL006188
6 · The paper itself

Abstract

Fluorescent RNA aptamers are tools for studying RNA localization and interactions in vivo. The photophysical properties of these in vitro selected RNAs should be characterized prior to cellular imaging experiments. Here, we describe the process of determining the fluorophore affinity, fluorescence enhancement, and fluorescence lifetime(s) of the Mango-III fluorescence turn-on aptamer. Parameters determined through these protocols will aid in establishing conditions for live-cell imaging.

Indexed as

Aptamers, NucleotideFluorescenceFluorescent DyesRNAAptamers, NucleotideFluorescent DyesRNAFluorescenceRiboswitchesRNA aptamersRNA imagingRNA localization

Identifiers

PMID36227560
PMCPMC9812286

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.