Evidence map›Paper›PMID 36227487›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Characterizing Properties of Biomolecular Condensates Below the Diffraction Limit In Vivo.

Ganesh Pandey, Alisha Budhathoki, Jan-Hendrik Spille

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
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  5. Article
  6. Using single molecule imaging to explore intracellular heterogeneity.The international journal of biochemistry & cell biology · 2023
    Review
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Ganesh PandeyDepartment of Physics, University of Illinois at Chicago, Chicago, IL, USA.
Alisha BudhathokiDepartment of Physics, University of Illinois at Chicago, Chicago, IL, USA.
Jan-Hendrik SpilleDepartment of Physics, University of Illinois at Chicago, Chicago, IL, USA. jhspille@uic.edu.

Funding

Igh locus function in immunosenescent miceR21AI159626 · NIAID · UNIVERSITY OF ILLINOIS AT CHICAGO · PI KENTER, AMY L · 2021 to 2022
$429k
NIAID NIH HHS R21 AI159626
6 · The paper itself

Abstract

Fluorescence microscopy assays enable the investigation of endogenous biomolecular condensates directly in their cellular context. With appropriate experimental designs, these assays yield quantitative information on condensate material properties and inform on biophysical mechanisms of condensate formation. Single-molecule super-resolution and tracking experiments grant access to the smallest condensates and early condensation stages not resolved by conventional imaging approaches. Here, we discuss considerations for using single-molecule assays to extract quantitative information about biomolecular condensates directly in their cellular context.

Indexed as

Biomolecular CondensatesMicroscopy, FluorescenceLiquid-liquid phase separationMultiphase assemblySingle-molecule trackingSuper-resolution microscopy

Identifiers

PMID36227487

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.