Evidence map›Paper›PMID 36225373›Full record

ArticleFrontiers in microbiology2022

No time to die: Comparative study on preservation protocols for anaerobic fungi.

Julia Vinzelj, Akshay Joshi, Diana Young, Ljubica Begovic, Nico Peer, Lona Mosberger, Katharina Cécile Schmid Luedi, Heribert Insam, Veronika Flad, Magdalena Nagler and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Description ofInternational journal of systematic and evolutionary microbiology · 2026
    Article
  2. Description ofbioRxiv : the preprint server for biology · 2025
    Article
  3. International journal of systematic and evolutionary microbiology · 2025
    Article
  4. Article
  5. Review
  6. Mycologia
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Julia VinzeljDepartment of Microbiology, University of Innsbruck, Innsbruck, Austria.
Akshay JoshiDepartment of Microbiology, University of Innsbruck, Innsbruck, Austria.
Diana YoungMicro- and Molecular Biology, Central Department for Quality Assurance and Analytics, Bavarian State Research Center for Agriculture, Freising, Germany.
Ljubica BegovicDepartment of Microbiology, University of Innsbruck, Innsbruck, Austria.
Nico PeerDepartment of Microbiology, University of Innsbruck, Innsbruck, Austria.
Lona MosbergerInstitute of Chemistry and Biotechnology, Biocatalysis and Process Technology Unit, Zurich University of Applied Sciences, Wäedenswil, Switzerland.
Katharina Cécile Schmid LuediInstitute of Chemistry and Biotechnology, Biocatalysis and Process Technology Unit, Zurich University of Applied Sciences, Wäedenswil, Switzerland.
Heribert InsamDepartment of Microbiology, University of Innsbruck, Innsbruck, Austria.
Veronika FladMicro- and Molecular Biology, Central Department for Quality Assurance and Analytics, Bavarian State Research Center for Agriculture, Freising, Germany.
Magdalena NaglerDepartment of Microbiology, University of Innsbruck, Innsbruck, Austria.
Sabine Marie PodmirsegDepartment of Microbiology, University of Innsbruck, Innsbruck, Austria.

Funding

Austrian Science Fund FWF I 3808
6 · The paper itself

Abstract

Anaerobic fungi (AF, phylum Neocallimastigomycota) are best known for their ability to anaerobically degrade recalcitrant lignocellulosic biomass through mechanic and enzymatic means. While their biotechnological potential is well-recognized, applied research on AF is still hampered by the time-consuming and cost-intensive laboratory routines required to isolate, maintain, and preserve AF cultures. Reliable long-term preservation of specific AF strains would aid basic as well as applied research, but commonly used laboratory protocols for AF preservation can show erratic survival rates and usually exhibit only moderate resuscitation success for up to one or two years after preservation. To address both, the variability, and the preservation issues, we have set up a cross-laboratory, year-long study. We tested five different protocols for the preservation of AF. The experiments were performed at three different laboratories (Austria, Germany, Switzerland) with the same three morphologically distinct AF isolates (

Indexed as

anaerobic fungicryopreservationculture preservationlong-term storageNeocallimastigomycotapreservation techniquesresting stagesshort-term storage

Identifiers

PMID36225373
PMCPMC9549207

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.