Evidence map›Paper›PMID 36220098›Full record

ArticleNeuron2022

In situ cell-type-specific cell-surface proteomic profiling in mice.

S Andrew Shuster, Jiefu Li, URee Chon, Miley C Sinantha-Hu, David J Luginbuhl, Namrata D Udeshi, Dominique Kiki Carey, Yukari H Takeo, Qijing Xie, Chuanyun Xu and 5 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuron, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.

0numbers the graph read from it
0cells of the map it votes in
41citing papers in PubMed
7.0field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

41 citing papers in PubMed, 66 citations in OpenAlex.

  1. Review
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  9. Fibro-adipogenic progenitor cells from murine SMA muscles are intrinsically adipogenic.Proceedings of the National Academy of Sciences of the United States of America · 2026
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  17. bioRxiv : the preprint server for biology · 2025
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  19. Cholinergic Waves Have a Modest Influence on the Transcriptome of Retinal Ganglion Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 3 institutions in 1 country.

S Andrew ShusterDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA; Neurosciences Program, Stanford University, CA 94305, USA.
Jiefu LiDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
URee ChonDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA; Neurosciences Program, Stanford University, CA 94305, USA.
Miley C Sinantha-HuDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
David J LuginbuhlDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Namrata D UdeshiThe Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Dominique Kiki CareyThe Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Yukari H TakeoDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Qijing XieDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA; Neurosciences Program, Stanford University, CA 94305, USA.
Chuanyun XuDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
D R ManiThe Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Shuo HanDepartments of Genetics, Biology, and Chemistry, Chan Zuckerberg Biohub, Stanford University, Stanford, CA 94305, USA.
Alice Y TingDepartments of Genetics, Biology, and Chemistry, Chan Zuckerberg Biohub, Stanford University, Stanford, CA 94305, USA.
Steven A CarrThe Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Liqun LuoDepartment of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA. Electronic address: lluo@stanford.edu.
Howard Hughes Medical Institute · USBroad Institute · USChan Zuckerberg Initiative (United States) · US

Funding

Training Program in Basic NeuroscienceT32MH020016 · NIMH · STANFORD UNIVERSITY · PI Justin L Gardner, Merritt C Maduke · 1997 to 2026
$16.2M
Mapping protein communication between organs in homeostasis and diseaseR01DK121409 · NIDDK · HARVARD MEDICAL SCHOOL · PI CARR, STEVEN A, MCMAHON, ANDREW P. · 2018 to 2022
$8.3M
Mosaic Analysis with Double Markers in MiceR01NS050835 · NINDS · STANFORD UNIVERSITY · PI LIQUN LUO · 2005 to 2026
$7.3M
Integrated high throughput proteogenomic data analysis center for CPTACU24CA210979 · NCI · BROAD INSTITUTE, INC. · PI BIRGER, CHET, GETZ, GAD A · 2016 to 2020
$4.4M
Neuromodulation of Brain StatesR01NS104698 · NINDS · STANFORD UNIVERSITY · PI LUO, LIQUN · 2018 to 2022
$3.3M
NCI NIH HHS U24 CA210979NIDDK NIH HHS R01 DK121409NIMH NIH HHS T32 MH020016NINDS NIH HHS R01 NS050835NINDS NIH HHS R01 NS104698
6 · The paper itself

Abstract

Cell-surface proteins (CSPs) mediate intercellular communication throughout the lives of multicellular organisms. However, there are no generalizable methods for quantitative CSP profiling in specific cell types in vertebrate tissues. Here, we present in situ cell-surface proteome extraction by extracellular labeling (iPEEL), a proximity labeling method in mice that enables spatiotemporally precise labeling of cell-surface proteomes in a cell-type-specific environment in native tissues for discovery proteomics. Applying iPEEL to developing and mature cerebellar Purkinje cells revealed differential enrichment in CSPs with post-translational protein processing and synaptic functions in the developing and mature cell-surface proteomes, respectively. A proteome-instructed in vivo loss-of-function screen identified a critical, multifaceted role for Armh4 in Purkinje cell dendrite morphogenesis. Armh4 overexpression also disrupts dendrite morphogenesis; this effect requires its conserved cytoplasmic domain and is augmented by disrupting its endocytosis. Our results highlight the utility of CSP profiling in native mammalian tissues for identifying regulators of cell-surface signaling.

Indexed as

MammalsProteomicsAnimalsMicecell-surface proteincell-surface proteomecell-surface signalingdendrite morphogenesisdiscovery proteomicsproximity labelingPurkinje cell

Identifiers

PMID36220098
PMCPMC9742329
OpenAlexW4304014316

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.